A quantitative look at the multitudinous benefits of CJC-1295 w/Dac & IGF-1 levels
As biology and the art of science blast through new discoveries and breakthroughs at a pace never seen before, NEW HRT/TRT and other hormone therapeutic protocols are being conducted and shining while delivering results that are making scientist eager to press forward for more data..The truth is science is now truly just grasping an understanding while considering greater possibilities, capabilities with astonishing data..Science knows more about the cosmos than they do with hormones, we're merely scratching the surface and there's always newer data/clinical studies out-dating an others contradicting each other and so on, but its only getting better and more advanced citing new data that is capturing the attention of scientist world wide..
A Few years back I decided to utilize the poor mans HGH option, known as peptides.. The results were not only surprising, but the blood work was stellar when it concerned my IGF-1 levels, surpassing all best serum scores I personally have ever seen or had..
My protocol wasn't HGH, It was CJC dac and MK677 & GHRP-6, scoring low 600's..
As we often see people attempting to re-engineer the wheel, with reports of "the latest new trend" that is spouted through bro-science, these fads seem to come in with blinding speeds, and often fade away soon after..
However, there's some real truth behind some of these findings citing new data never seen before, and these can be supported with actual studies that are currently taken place world wide, so keep on with the personal science experiments, as we are truly our very own science experiment..
So, Let's take a quantitative look at the multitudinous benefits that peptides may actually posses!!!
In the meantime Stay well,
Vision
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Prolonged Stimulation of Growth Hormone (GH) and Insulin-Like Growth Factor I Secretion by CJC-1295, a Long-Acting Analog of GH-Releasing Hormone, in Healthy Adults
Sam L. Teichman Ann Neale Betty Lawrence Catherine Gagnon Jean-Paul Castaigne Lawrence A. Frohman
The Journal of Clinical Endocrinology & Metabolism, Volume 91, Issue 3, 1 March 2006, Pages 799–805, https://doi.org/10.1210/jc.2005-1536 Published: 01 March 2006
Abstract
Context: Therapeutic use of GHRH to enhance GH secretion is limited by its short duration of action.
Objective: The objective of this study was to examine the pharmacokinetic profile, pharmacodynamic effects, and safety of CJC-1295, a long-acting GHRH analog.
Design: The study design was two randomized, placebo-controlled, double-blind, ascending dose trials with durations of 28 and 49 d.
Setting: The study was performed at two investigational sites.
Participants: Healthy subjects, ages 21–61 yr, were studied.
Interventions: CJC-1295 or placebo was administered sc in one of four ascending single doses in the first study and in two or three weekly or biweekly doses in the second study.
Main Outcome Measures: The main outcome measures were peak concentrations and area under the curve of GH and IGF-I; standard pharmacokinetic parameters were used for CJC-1295.
Results: After a single injection of CJC-1295, there were dose-dependent increases in mean plasma GH concentrations by 2- to 10-fold for 6 d or more and in mean plasma IGF-I concentrations by 1.5- to 3-fold for 9–11 d. The estimated half-life of CJC-1295 was 5.8–8.1 d. After multiple CJC-1295 doses, mean IGF-I levels remained above baseline for up to 28 d. No serious adverse reactions were reported.
Conclusions: Subcutaneous administration of CJC-1295 resulted in sustained, dose-dependent increases in GH and IGF-I levels in healthy adults and was safe and relatively well tolerated, particularly at doses of 30 or 60 μg/kg. There was evidence of a cumulative effect after multiple doses. These data support the potential utility of CJC-1295 as a therapeutic agent.
Topic:
Issue Section:
Endocrine Care
THE USE OF GH for the treatment of children with impaired linear growth has been accepted as an important therapeutic modality for more than 50 yr (1). An unlimited supply of the hormone, made possible by the availability of recombinant GH in the 1980s, permitted expansion of the target population to include GH-deficient adults. Most adults receiving GH today have primary pituitary disease with impaired GH secretory capacity. However, most children being treated with GH have no evidence of pituitary disease and are believed to have an impaired hypothalamic signaling mechanism due to a GHRH neurosecretory dysfunction. GH has also been used for therapy of disorders in children and adults in which pituitary function is either intact or only slightly impaired, such as chronic renal failure and Turner syndrome (in children) and HIV-related wasting and lipodystrophy and burn therapy (in adults).
In patients with intact pituitary function, there has been interest in the use of GHRH rather than GH in the hope of producing a more physiological pattern of tissue exposure to GH than occurs by a single daily injection of the hormone. In fact, several studies in both children and adults have suggested that comparable or near-comparable results can be achieved with GHRH therapy (2–4).
A major limitation in the use of GHRH for therapy, however, is its short half-life. Native GHRH, a 44-amino acid peptide, has a half-life of 7 min (5), which is even shorter than that of GH (12 min) (6), necessitating daily or even more frequent injections. Polyethylene glycol-conjugated GHRH has been studied in an effort to overcome this limitation (7).
A synthetically modified form of GHRH has been linked to a reactive chemical that enables binding to endogenous serum albumin after sc administration. The chemical structure of this compound, drug affinity complex-GH-releasing factor (DAC-GRF; CJC-1295, ConjuChem, Inc., Montréal, Canada) is shown in Fig. 1. The core therapeutic moiety is GHRH-(1–29)NH[SUB]2[/SUB], which contains the full biological activity of GHRH-(1–44)NH[SUB]2[/SUB] modified by substitution of four amino acids that serve to render the compound more resistant to proteolytic cleavage (herein called GRF). GRF is linked by the amino acid, lysine, to a reactive chemical [maleimidoproprionic acid (MPA)] that binds to unpaired thiol (sulfhydryl) groups. The predominant free thiol group available for binding after parenteral administration is the single unpaired cysteine (cysteine 34) in serum albumin. At least 90% of CJC-1295 binds covalently to albumin in this fashion, with trace amounts found bound to fibrinogen and IgG. No other chemical species have been found bound to DAC-GRF after administration (data on file, ConjuChem, Inc.). This binding extends the half-life of the active pharmacophore, resulting in a markedly prolonged duration of action in several animal species (8). Moreover, studies in both dogs and pigs indicate that physiological GH secretion is maintained, and IGF-I levels are enhanced for several days after a single administration.
We assessed the safety, tolerability, pharmacokinetic profile, and effect of CJC-1295 on circulating concentrations of GH and IGF-I in two randomized, placebo-controlled, double-blind, dose-escalating studies in healthy adult subjects.
Fig. 1.
View largeDownload slide
Chemical structure of the CJC-1295 (DAC-GRF). The core therapeutic moiety is a tetrasubstituted GHRH-(1–29)NH[SUB]2[/SUB]. The substituted amino acids are shown in italics. The linker is lysine, and the reactive chemical is maleimidoproprionic acid that binds covalently to the single unpaired cysteine (cysteine 34) in serum albumin.
Subjects and Methods
The subjects consisted of healthy men and women, ages 21–61 yr, with a body mass index of 30 kg/m[SUP]2[/SUP] or less and IGF-I levels in the normal range for age and gender. Appropriately constituted independent ethics committees reviewed and approved each of the studies, and written informed consent of all subjects was obtained before participation.
Study design
Study 1 was an ascending, randomized, double-blind, placebo-controlled single-dose trial performed at SFBC International, Inc. (Miami, FL). Study 2 was an ascending, randomized, double-blind, placebo-controlled multiple-dose trial performed at Kendle International BV (Utrecht, The Netherlands).
In study 1, four sequential, dose-escalation groups were evaluated. These dose levels were 30 μg/kg (n = 6, five active and one placebo), 60 μg/kg (n = 6, five active and one placebo), 125 μg/kg (n = 6, five active and one placebo), and 250 μg/kg (n = 6, five active and one placebo). An additional cohort of 18 subjects (15 active and three placebo) subsequently received 125 μg/kg.
Serum GH was measured on d 0 at 60, 30, and 15 min before study drug dosing; at 15, 30, and 60 min and 2, 3, 4, 6, 8, 10, 12, and 24 h after dosing; and then every 8 h on d 2–3, then daily on d 4, 5, 6, 7, 9, 11, 14, 21, and 28. Serum IGF-I and CJC-1295 were measured on d 0, 1, 2, 3, 4, 5, 6, 7, 9, 11, 14, 21, and 28.
In study 2, 24 subjects were enrolled in one of four sequential dosing cohorts. Group 1 (n = 6, five active and one placebo) received two injections of 30 μg/kg (d 0 and 14), group 2 (n = 6, five active and one placebo) received two injections of 60 μg/kg (d 0 and 14), group 3 (n = 6, five active and one placebo) received three injections of 30 μg/kg (d 0, 7, and 14), and group 4 (n = 6, five active and one placebo) received three injections of 20 μg/kg (d 0, 7, and 14). Sample collection was similar to that in study 1, with the addition of more frequent pre- and postinjection sampling on d 7 (groups 3 and 4 only) and 14 as well as a final sample collection on d 49 in all subjects.
Serial clinical evaluations (vital signs, adverse events, and physical examination) and laboratory safety assessments (serum chemistry, hematology, and urinalysis) were performed ending on d 28 in study 1 and on d 49 in study 2.
Laboratory methods
GH.
Serum GH was measured by a double antibody RIA (Esoterix Laboratory Services, Inc., Calabasas Hills, CA). The assay sensitivity was 0.3 ng/ml, and the coefficient of variation was 10%.
IGF-I.
Serum IGF-I was measured by a double antibody RIA by Esoterix Laboratory Services, Inc., after ethanol extraction and with the addition of IGF-2 as a blocking agent. The assay sensitivity was 10 ng/ml, and the coefficient of variation was 5.4%. Normal ranges for the assay are age and gender adjusted.
Other hormones.
Serum cortisol, prolactin, TSH, and LH concentrations were measured in patients receiving 60 μg/kg CJC-1295 in study 1 by Esoterix Laboratory Services, Inc.
CJC-1295.
Plasma CJC-1295 concentrations were measured by RIA at PPD Development, LP (Richmond, VA), using a rabbit anti-(tetra-substituted)GRF-(1–29) coupled to keyhole limpet hemocyanin and radioiodinated GRF-(1–29). The antibody exhibited 100% cross-reactivity with albumin-bound DAC of the tetrasubstituted GRF-(1–29). There was no cross-reactivity with native GRF-(1–29), native GRF-(3–29), or the DAC of the fragment 12–29 of the tetrasubstituted GRF. There was 25% cross-reactivity with free (nonalbumin-bound) tetrasubstituted GRF-(1–29). The lower limit of detection was 0.2 nmol/liter, and the mean intra- and interassay coefficients of variation were 5.5% and 8.2%, respectively.
Pharmacokinetic analysis.
Pharmacokinetic parameters [peak plasma concentrations (C[SUB]max[/SUB]), time to peak plasma concentrations (T[SUB]max[/SUB]), and area under the curve (AUC)] of CJC-1295, GH, and IGF-I were calculated from the concentration vs. time values for each patient using a compartment model in the single-dose study (WinNonlin Professional version 4.1, Pharsight Corp., Mountain View CA) and a noncompartmental model in the multiple-dose study (WinNonlin Professional version 4.0.1).
Antibody formation.
A validated immunoradiometric assay was used to determine the presence of antibodies to CJC-1295. The anti-CJC-1295 antibody was raised in rabbits by immunization with a CJC-1295 analog [the tetrasubstituted GRF-(1–29)] to which a cysteine residue was added at position 30 to permit direct conjugation to keyhole limpet hemocyanin to make the molecule more immunogenic. This antibody was also used in the assay for plasma CJC-1295 concentrations. In this immunoradiometric assay, tubes are coated with CJC-1295 bound to inactivated MPA. Test samples or affinity-purified rabbit anti-CJC-1295 antibody controls in human serum were added. After incubation, tubes were washed and [[SUP]125[/SUP]I]protein LA (Sigma-Aldrich, St. Louis, MO) was added. After incubation, tubes were washed again, radioactivity was determined in a γ-counter, and the specific binding of the samples was calculated.
Statistical analysis
Mean and variance estimates were calculated for all pharmacokinetic parameters by dose group. C[SUB]max[/SUB] and AUC to the last sampling time (AUC[SUB]t[/SUB]) were log transformed before analysis, and AUC values were calculated using the linear trapezoidal rule. Differences in GH and IGF-I levels and AUC between groups were compared by ANOVA and/or one-tailed t test; P < 0.05 was considered significant. All statistical analyses were performed using SAS version 8.02 (SAS Institute, Cary, NC).
Because all enrolled subjects received at least one dose of the study drug, all available data are included in analyses of safety, pharmacokinetic, and pharmacodynamic parameters. No effort to estimate missing data was made, with the exception of AUC calculations.
Subjects receiving placebo in all dosing groups in each study were pooled for comparison with groups treated with active drug.
Results
Subject characteristics and disposition
The distribution of subjects in the study groups by age and gender is shown in Table 1. The mean overall age of subjects in the two studies was 49.3 ± 1.1 (±se) yr (range, 21–61 yr), and 44% of subjects were men. Although the dosing cohort groups in both studies were generally similar in mean age, gender, height, weight, and BMI, subjects in group 2 in study 2 were younger (mean age, 33 yr), and 80% were men. Two subjects in study 1 (both randomized to the 125 μg/kg dosing cohort) and one subject in study 2 (in group 2) discontinued the study prematurely. The subjects in study 1 withdrew 5 and 22 d after dosing because of mild injection site reactions. The subject in study 2 withdrew 6 d after experiencing multiple mild adverse effects following a single injection of 30 μg/kg.
TABLE 1.
Study drug doses and regimens with subject information
[TABLE="class: cms_table"]
<tbody>[TR]
[TH="align: center"][/TH]
[TH="align: center"]Total no.[/TH]
[TH="align: center"]Age ± sd[/TH]
[TH="align: center"]No. of men[/TH]
[TH="align: center"]No. of women[/TH]
[TH="align: center"]No. of doses[/TH]
[TH="align: center"]Dosing days[/TH]
[/TR]
[TR]
[TD]Study 1[/TD]
[TD][/TD]
[TD][/TD]
[TD][/TD]
[TD][/TD]
[TD][/TD]
[TD][/TD]
[/TR]
[TR]
[TD] Dose[/TD]
[TD][/TD]
[TD][/TD]
[TD][/TD]
[TD][/TD]
[TD][/TD]
[TD][/TD]
[/TR]
[TR]
[TD] 30 μg/kg[/TD]
[TD]5[/TD]
[TD]55 ± 8[/TD]
[TD]2[/TD]
[TD]3[/TD]
[TD]1[/TD]
[TD][/TD]
[/TR]
[TR]
[TD] 60 μg/kg[/TD]
[TD]5[/TD]
[TD]53 ± 7[/TD]
[TD]2[/TD]
[TD]3[/TD]
[TD]1[/TD]
[TD][/TD]
[/TR]
[TR]
[TD] 125 μg/kg[/TD]
[TD]20[/TD]
[TD]50 ± 6[/TD]
[TD]7[/TD]
[TD]13[/TD]
[TD]1[/TD]
[TD][/TD]
[/TR]
[TR]
[TD] 250 μg/kg[/TD]
[TD]5[/TD]
[TD]53 ± 3[/TD]
[TD]1[/TD]
[TD]4[/TD]
[TD]1[/TD]
[TD][/TD]
[/TR]
[TR]
[TD] Pooled placebo[/TD]
[TD]7[/TD]
[TD]52 ± 7[/TD]
[TD]3[/TD]
[TD]4[/TD]
[TD]1[/TD]
[TD][/TD]
[/TR]
[TR]
[TD]Study 2[/TD]
[TD][/TD]
[TD][/TD]
[TD][/TD]
[TD][/TD]
[TD][/TD]
[TD][/TD]
[/TR]
[TR]
[TD] Dose[/TD]
[TD][/TD]
[TD][/TD]
[TD][/TD]
[TD][/TD]
[TD][/TD]
[TD][/TD]
[/TR]
[TR]
[TD] 20 μg/kg[/TD]
[TD]5[/TD]
[TD]43 ± 14[/TD]
[TD]3[/TD]
[TD]2[/TD]
[TD]3[/TD]
[TD]d 0, 7, 14[/TD]
[/TR]
[TR]
[TD] 30 μg/kg[/TD]
[TD]5[/TD]
[TD]33 ± 15[/TD]
[TD]4[/TD]
[TD]1[/TD]
[TD]3[/TD]
[TD]d 0, 7, 14[/TD]
[/TR]
[TR]
[TD] 30 μg/kg[/TD]
[TD]5[/TD]
[TD]40 ± 11[/TD]
[TD]3[/TD]
[TD]2[/TD]
[TD]2[/TD]
[TD]d 0, 14[/TD]
[/TR]
[TR]
[TD] 60 μg/kg[/TD]
[TD]5[/TD]
[TD]57 ± 1[/TD]
[TD]2[/TD]
[TD]3[/TD]
[TD]2[/TD]
[TD]d 0, 14[/TD]
[/TR]
[TR]
[TD] Pooled placebo[/TD]
[TD]4[/TD]
[TD]51 ± 13[/TD]
[TD]2[/TD]
[TD]2[/TD]
[TD]2 or 3[/TD]
[TD]d 0, 14, or d 0, 7, 14[/TD]
[/TR]
</tbody>[/TABLE]
View Large
Plasma CJC-1295 concentrations
In study 1, the mean C[SUB]max[/SUB] of CJC-1295 increased with dose, with values of 2.17, 5.19, 8.16, and17.1 nmol/liter in the 30, 60, 125, and 250 μg/kg single-dose groups, respectively (Fig. 2). Maximum drug concentrations were reached between 1 and 1.5 h in all groups. The mean AUC[SUB]t[/SUB] in the 30, 60, 125, and 250 μg/kg dose groups were 143, 355, 669, and 1276 h·nmol/liter, respectively, with dose-proportionality observed in the 60, 125, and 250 μg/kg dose groups. Terminal elimination rate, systemic clearance, and volume of distribution gradually increased with dose. The mean half-life ranged from 5.8–8.1 d; mean systemic clearances were 0.04, 0.04, 0.05, and 0.05 liters/h/kg, and mean volumes of distribution were 8.1, 9.7, 11.6, and 13.8 liter/kg in the four dose groups, respectively.
Fig. 2.
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Plasma disappearance curves of CJC-1295 after a single sc injection. Shown are the mean ± sd half-life. Drug concentrations were generally measurable for at least 12–14 d after injection.
In study 2, maximum CJC-1295 plasma concentrations were 11–32% higher after the injection on d 7 than on d 0 in the two groups that received weekly injections (data not shown). After the d 14 injection, maximum CJC-1295 concentrations were 29–70% higher than those on d 0 in all four groups. Similar increases occurred in AUC[SUB]0–24 h[/SUB] on d 7 (12% and 15%) in groups 3 and 4 and in all four dosing groups on d 14 (31–57%). The 0–24 h AUC values were dose dependent.
Maximum drug concentrations were typically reached within 0.5–2.0 h after injection, but there was a high degree of individual variability. The variability increased with subsequent doses, but did not appear to be dose dependent. Drug concentrations were measurable for 10–14 d. In subjects in whom samples were obtained for up to 14 d, the mean estimated half-life of CJC-1295 ranged from 5.4–9.2 d, and the mean clearance was between 1.1 and 3.3 liters/h. The pharmacokinetic parameters were independent of body weight.
Serum GH concentrations
In study 1, mean preinjection GH concentrations ranged from 0.7–1.1 ng/ml. Mean GH concentrations increased by 2- to 10-fold after single-dose injection of CJC-1295 through d 6 (Fig. 3A). In contrast, mean GH concentrations remained stable in the placebo group. The mean GH AUC[SUB]0–7 d[/SUB] values were elevated in a dose-dependent manner, although only the groups receiving 60, 125, and 250 μg/kg had significant increases compared with the placebo group (Fig. 3B).
Fig. 3.
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GH responses to a single sc injection of CJC-1295. A, Serum GH concentrations (mean ± sd) are shown and suggest that pulsatile hormone secretion is maintained. B, Mean GH AUC[SUB]0–7 d[/SUB], expressed as a percent increase over placebo. *, P < 0.05 vs. placebo. Shown are the mean ± sd. Mean maximum concentrations of GH were 6.6, 9.6, 9.9, and 13.3 ng/ml in the 30, 60, 125, and 250 μg/kg groups; mean AUC were 758, 969, 977, and 1370 ng/ml·h, respectively.
The median peak GH level occurred within 1 h in all dosing groups in both studies, with the exception of the group receiving a single 250 μg/kg dose of CJC-1295, in which the median peak GH level occurred at 4 h. The mean peak GH levels were more variable and occurred 0.5–4 h after dosing. The variability was neither dose dependent nor progressive.
Serum IGF-I concentrations
Mean preinjection IGF-I concentrations ranged from 123–152 ng/ml in study 1. IGF-I levels remained elevated compared with baseline for 9–11 d after a single injection of CJC-1295 in all dosing groups in study 1, and mean levels increased by 0.5- to 3-fold over baseline (Fig. 4A). Mean IGF-I AUC[SUB]0–7 d[/SUB] values were elevated in a dose-dependent manner, reaching statistical significance compared with baseline in the groups receiving 60, 125, and 250 μg/kg (Fig. 4B). IGF-I levels exceeded the age- and gender-adjusted normal ranges only in subjects receiving 250 μg/kg CJC-1295. In contrast, mean IGF-I levels in the placebo group remained relatively stable during the same period. The time to peak IGF-I levels was dose dependent, occurring 2–3 d after administration of the lowest three doses, but not until 4 d after the highest dose. IGF-I levels remained at a plateau for up to 7 d, after which they gradually declined toward baseline. IGF-I levels remained elevated for at least 2 wk after injection in patients receiving the two highest doses.
Fig. 4.
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IGF-I responses to a single sc injection of CJC-1295. A, Serum IGF-I concentrations (mean ± sd) are shown. B, Mean IGF-I AUC[SUB]0–7 d[/SUB], expressed as a percent increase over placebo. *, P < 0.05 vs. placebo. Shown are the mean ± sd. The shaded area marked is the upper limit of normal (U.L.N.) for age- and gender-matched cohorts. Mean maximum concentrations of IGF-I were 232, 319, 328, and 435 ng/ml in the 30, 60, 125, and 250 μg/kg groups; mean AUC were 91, 127, 119, and 172 μg/ml·h, respectively.
In study 2, mean IGF-I levels increased within 8 h of CJC-1295 injection and remained above baseline levels through d 28 (Fig. 5). Mean IGF-I values remained elevated above baseline before the second and/or third doses in all CJC-1295-treated groups, although this reached significance only for group 4 (P < 0.03 for group 4 and P < 0.07 for group 3, by paired t test). Maximum IGF-I levels after the second and/or third injections were progressively greater than after the initial injection (data not shown). In addition, the T[SUB]max[/SUB] for IGF-I was progressively shorter after subsequent injections (data not shown).
Fig. 5.
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IGF-I responses to multiple sc injections of CJC-1295. Serum IGF-I concentrations (mean ± se) are shown. Arrows indicate days of injection. A, Changes in serum IGF-I levels after three weekly injections of CJC-1295 or placebo. B, Changes in serum IGF-I levels after two biweekly injections of CJC-1295 or placebo. In both A and B, for d 0–7 and 14–21, the entire pooled placebo group (n = 4) is plotted, because all these subjects received placebo injections on d 0 and 14. In A, for the period from d 7–14, only the two subjects who received placebo injections on d 7 are plotted (i.e. three weekly injections). In B, for the period from d 7–14, only the two subjects who received placebo injections on d 0 and 14 are plotted (i.e. two biweekly injections).
Mean AUC[SUB]0–7 d[/SUB] and AUC[SUB]0–14 d[/SUB] for IGF-I were significantly increased in group 2 (60 μg/kg) compared with group 1 (30 μg/kg; P = 0.041 and P = 0.043, respectively) and were significantly greater in both groups compared with the placebo group (P = 0.003 and P = 0.005, respectively). Mean AUC[SUB]0–7 d[/SUB], AUC[SUB]7–14 d[/SUB], and AUC[SUB]14–21 d[/SUB] were all significantly higher in group 3 (30 μg/kg) than in group 4 (20 μg/kg; P = 0.010, P = 0.020, and P = 0.026, respectively).
Other than age, no significant predictors of IGF-I or GH response, including gender, baseline weight or body mass index, and lipid or glucose levels, were observed.
Other hormonal effects
There were no significant increases in serum cortisol, prolactin, TSH, or LH levels after a single injection of CJC-1295 (60 μg/kg), thus confirming the specificity of CJC-1295 for GH secretion (data not shown).
Safety
In the single-dose study, adverse events were reported in 33 (94%) vs. two (29%) subjects in the active and placebo groups, respectively; all were of mild to moderate severity, and none required medical intervention to resolve. Injection site reactions (irritation, erythema, induration, pain, or itching) occurred transiently (up to several hours) in approximately 70% of subjects receiving CJC-1295 and rarely in subjects receiving placebo. Injection site reactions tended to be more severe and/or prolonged after higher doses, with residual induration lasting up to 5 d. No local reaction exceeded 10 cm in diameter, and all resolved spontaneously. Transient urticarial rashes at the injection site occurred in almost 30% of subjects and were not dose related. Other adverse events reported in actively treated subjects included headache (63%), diarrhea (43%), and systemic vasodilatory reactions (flushing, warmth, and transient hypotension; 30%); all were more common at higher doses (125 or 250 μg/kg). Headache and diarrhea occurred occasionally at various times during the 7 d after dosing. Of these adverse events, only headache occurred in the placebo-treated group (14%). Overall, the adverse events observed at 250 μg/kg were moderate in severity and resolved spontaneously after a few hours, whereas the 125 μg/kg dose of CJC-1295 was considered well tolerated.
Injection site reactions (irritation, erythema, induration, pain, or itching) were reported in all actively treated subjects in study 2; all were mild in severity. Reactions were up to 10 cm in diameter. Mild injection site erythema (<2–3 cm) was reported in three of four placebo-treated subjects (75%) as well as induration and urticaria (<1–2 cm; 25%). Flushing occurred only in actively treated subjects, occurred within 30 min of injection, and resolved within 1–2 h. The incidence of flushing was dose dependent, with an incidence of 40% after low-dose and 100% after high-dose injections.
Other adverse events included transient loose stools/diarrhea (45% and 100% incidence in the 125 and 250 μg/kg groups, respectively), headache (non-dose dependent and ranging from 20–80% depending on the dose group), and nausea or abdominal pain (20%). Of these adverse events, only headache occurred in the placebo-treated group (14% in study 1 and 50% in study 2).
The incidence and severity of all adverse events were gender independent. There were no consistent changes in blood or urine laboratory values, including glucose levels and liver function studies, or in electrocardiographic findings in either study. No significant antibody formation was detected in subjects who received the active study drug.
A single subject in study 2 reported mild, transient (resolving fully and spontaneously in <24 h) involuntary leg muscle contractions and some loss of coordination after receiving the second biweekly injection of 30 μg/kg. Two subjects experienced transient dizziness and hypotension after the first injection of 30 μg/kg that resolved spontaneously and did not recur after subsequent injections of CJC-1295.
Discussion
This report describes the safety, pharmacokinetic profile, and pharmacodynamic effects of CJC-1295, a synthetic analog of GHRH that permanently and covalently binds to serum albumin after administration. Results of the single-dose and multiple-dose studies demonstrate a prolonged half-life of CJC-1295 (∼6–8 d) after sc administration, with measurable drug concentrations for 10–13 d after single or multiple doses. In addition, there was clear evidence of a dose-responsive and sustained biological effect, with elevated GH and IGF-I serum concentrations persisting for at least 6 and 14 d, respectively, after single doses of CJC-1295. In the multiple-dose study, there was a cumulative effect after two or three injections of CJC-1295 administered weekly or biweekly, with elevated levels of both GH and IGF-I above baseline on d 14 in most subjects. CJC-1295 was safe and generally well tolerated, particularly at doses of 30 and 60 μg/kg.
Treatment with human GH typically consists of a single daily injection of the hormone, resulting in transient supraphysiological levels, followed by a decline to baseline. However, failure to mimic the physiological pulsatile nature of GH secretion may preclude optimal therapeutic effects and may contribute to some of the adverse effects that have been observed even in the presence of normal serum IGF-I levels. In contrast, injections or infusions of GHRH stimulate the pulsatile release of GH (9, 10), but the short plasma half-life (7 min) (5) renders GHRH impractical for therapeutic use. Therefore, the availability of a GHRH preparation with sustained effect has important therapeutic potential.
The half-life of CJC-1295, as predicted from preclinical animal studies (8), was substantially prolonged compared with that of native GHRH, ranging from 5.8–8.1 d in the single-dose study and from 5.4–9.2 d in the multiple-dose study. Maximum concentrations were typically reached within 2 h after injection and exhibited a slow exponential decrease over several days. The disappearance rates were not dose dependent, although serum CJC-1295 concentrations were proportional to the dose injected. In the multiple-dose study, C[SUB]max[/SUB] and AUC[SUB]0–24 h[/SUB] were 17% greater on d 7 than on d 0 and from 30–70% greater on d 14 than on d 0.
Administration of single doses of CJC-1295 resulted in a 2- to 10-fold increase in mean serum GH levels in all dosing groups, which was dose incremental and persisted for up to 6 d. Similarly, a dose-related increase in mean serum IGF-I levels was observed at all dose levels, ranging from 1.5- to 3-fold and persisting for up to 14 d. Administration of ascending multiple doses of CJC-1295 resulted in elevated levels of GH, similar to those observed after a single dose.
In contrast, elevations in IGF-I levels showed a progressive effect over time, particularly in subjects receiving CJC-1295 every 7 d. Results of the multiple-dose study suggest both a cumulative pharmacokinetic effect [i.e. persistence of elevated predose levels of IGF-I in all dosing groups except group 1 (i.e. two injections of 30 μg/kg)] and a pituitary priming effect (i.e. progressively greater C[SUB]max[/SUB] and progressively shorter T[SUB]max[/SUB] after serial dosing). The data indicate that a minimum dosing interval of 7 d appears reasonable. The most appropriate dosing interval will be determined based on actual efficacy and safety data from longer-term therapeutic studies in patients with various clinical conditions.
No serious adverse reactions were reported in either study. The most frequently reported adverse events in subjects receiving CJC-1295 were injection site reactions, consisting of transient pain, swelling, and induration that were sometimes accompanied by local urticaria. Injection site reactions tended to be more severe and/or prolonged at higher dose levels. Headache, diarrhea, and flushing were also observed, with occasional transient and mild hypotension, but occurred primarily at higher doses.
Adverse effects complicate the use of GH in the treatment of HIV-associated metabolic conditions such as wasting and lipodystrophy. Although increases in the daily dose of GH from 1 to 6 mg are associated with dose-responsive benefits (11–13), doses of 2–3 mg/d or greater are associated with edema, arthralgias, and glucose intolerance. These side effects can become dose limiting. In the current studies, none of the subjects experienced these adverse effects. Future clinical trials on this disorder will confirm whether the use of GHRH rather than GH will circumvent these problems, as has been suggested in recent publications (4, 14).
In summary, a single sc administration of CJC-1295 produced sustained elevations of serum GH and IGF-I levels in normal subjects for nearly 2 wk. Weekly or biweekly administration of CJC-1295 resulted in stimulation of GH and IGF-I secretion for at least 7 d. Both single and multiple doses of CJC-1295 over 2 wk were safe and generally well tolerated, particularly at doses of 30 and 60 μg/kg. Future studies are indicated to evaluate the clinical utility of treatment with CJC-1295 in patients with intact GH secretory capacity.
Read more below
- - - Updated - - -
Last but not least
Just some more information about CJC-1295; it's worth the time invested while reading..I've been saying for sometime now that peptides are the way to go.. For GHRP-6 it's not for the faint-hearted, if you struggle with BS stability I would not suggest it although a lot of peptides may give a "false sense" of going hypo (LBG), it mainly comes with the territory of GHRP-2/6..Although it's primarily a false sense it can feel and almost demonstrate just as equal or if not more server than a real instances of going hypo by ones own diet/exercise and or genetics and so on..Having carbs/food on hand can easily mitigate these sides for those that are sensitive, furthermore it can be capitalized on and taken advantage of for those that have a tendency to not have the urge or desire to eat, this onset may provide the ideal platform for having the need or desire to want to consume food, almost instantaneously for some..
The combo of the both have made rest and recovery a beautiful thing in respect to feeling sleepy and wanting to shut my eyes almost anywhere at any given time, not to the point where it hinders my daily life but if I wish to nap, I can, and I will wherever I wish and even a small nap at 10-20 mins I wake up feeling as if I was out for hrs, groggy, but rested and ready to pounce!
Scientific research CJC-1295
In the experiment of Ionescu M. et al. [5], men aged 20-40 years were given a single dose of CJC-1295 (60 or 90 microg / kg body weight CJC-1295), a blood sample was collected every 20 minutes. This means that for a person weighing 90 kg, 5400 mcg (5.4 mg) or 8100 mcg (8.1 mg) CJC-1295 was administered.
Results? After a single dose of CJC-1295:
In another study, Teichman SL. Both co-workers [1] lasted 28 or 49 days. Healthy people aged 21-61 participated in it.
After a single injection of CJC-1295 (30 or 60 microg / kg body weight CJC-1295), there were:
GHRH agents interact through other mechanisms and synergistically together with GHRP, causing a much stronger GH and IGF-1 burst.
This is clearly demonstrated by numerous scientific studies, including experiment carried out by Popovic V. et al. [2].
One of the studies reported:
What was the effect on growth hormone?
This study clearly shows that growth hormone releasing peptides should not be used alone, without the administration of, for example, GHRH analogs (tesamorelin, TH9507, CJC-1295, MOD GRF 1-29, MR-356, MR-361, JI-38, MR-356 403, MR-367).
Side effects of using CJC-1295:
Unfortunately, there is no information on this subject, it can be suspected that, like tesamorelin CJC-1295, it can trigger:
The website administrator recognizes the phenomenon of doping and the use of illicit substances in sport, and contrary to medical prescriptions, for extremely reprehensible, unfair and immoral. Any information contained in articles on doping and pharmacology can not be treated or served as instructions. The content of the articles presents only the information collected about the above topic. The use of doping carries the risk of losing health and in many cases even the loss of life.
Study below:
Prolonged stimulation of growth hormone (GH) and insulin-like growth factor I secretion by CJC-1295, a long-acting analog of GH-releasing hormone, in healthy adults.
Teichman SL1, Neale A, Lawrence B, Gagnon C, Castaigne JP, Frohman LA.
Author information
Abstract
CONTEXT:
Therapeutic use of GHRH to enhance GH secretion is limited by its short duration of action.
OBJECTIVE:
The objective of this study was to examine the pharmacokinetic profile, pharmacodynamic effects, and safety of CJC-1295, a long-acting GHRH analog.
DESIGN:
The study design was two randomized, placebo-controlled, double-blind, ascending dose trials with durations of 28 and 49 d.
SETTING:
The study was performed at two investigational sites.
PARTICIPANTS:
Healthy subjects, ages 21-61 yr, were studied.
INTERVENTIONS:
CJC-1295 or placebo was administered sc in one of four ascending single doses in the first study and in two or three weekly or biweekly doses in the second study.
MAIN OUTCOME MEASURES:
The main outcome measures were peak concentrations and area under the curve of GH and IGF-I; standard pharmacokinetic parameters were used for CJC-1295.
RESULTS:
After a single injection of CJC-1295, there were dose-dependent increases in mean plasma GH concentrations by 2- to 10-fold for 6 d or more and in mean plasma IGF-I concentrations by 1.5- to 3-fold for 9-11 d. The estimated half-life of CJC-1295 was 5.8-8.1 d. After multiple CJC-1295 doses, mean IGF-I levels remained above baseline for up to 28 d. No serious adverse reactions were reported.
CONCLUSIONS:
Subcutaneous administration of CJC-1295 resulted in sustained, dose-dependent increases in GH and IGF-I levels in healthy adults and was safe and relatively well tolerated, particularly at doses of 30 or 60 microg/kg. There was evidence of a cumulative effect after multiple doses. These data support the potential utility of CJC-1295 as a therapeutic agent.
Study Below:
Blocked growth hormone-releasing peptide (GHRP-6)-induced GH secretion and absence of the synergic action of GHRP-6 plus GH-releasing hormone in patients with hypothalamopituitary disconnection: evidence that GHRP-6 main action is exerted at the hypothalamic level.
Popovic V1, Damjanovic S, Micic D, Djurovic M, Dieguez C, Casanueva FF.
Author information
Abstract
GH-releasing peptide (GHRP-6; His-D Trp-Ala-Trp-D Phe-Lys-NH2) is a synthetic compound that releases GH in a specific and dose-related manner through mechanisms and a point of action that are mostly unknown but different from those of GHRH. In man, GHRP-6 is more efficacious than GHRH, and a striking synergistic action on GH release is observed when GHRP-6 and GHRH are administered simultaneously. Based on such a synergistic action, it has been hypothesized that GHRP-6 acts through a double mechanism by actions exerted both at the pituitary and hypothalamic levels. The aim of the present study was 2-fold: 1) to further characterize the mechanism of action and synergistic effects of GHRP-6; and 2) to study its action in patients with hypothalamopituitary disconnection. Twelve patients with different neuroendocrine pathologies leading to a state of hypothalamopituitary disconnection (functional stalk section) and 11 age- and sex-matched normal controls were studied. Each subject underwent 3 tests on separate occasions, being challenged with GHRH (100 micrograms, i.v.), GHRP-6 (90 micrograms, i.v.), or GHRH plus GHRP-6. GH was analyzed as the area under the curve (mean +/- SE, micrograms per L/120 min). In normal subjects GH secretion was 483.7 +/- 99.2 after GHRH, 1434.8 +/- 393.0 after GHRP-6, and 3771.5 +/- 399.6 after GHRH plus GHRP-6; the level of GH secreted after GHRH plus GHRP-6 treatment was significantly (P < 0.05) higher than after the arithmetic sum of GH levels after both compounds administered separately. In the group of patients with hypothalamopituitary disconnection, the level of GH secreted after GHRH was similar to that in controls (423.4 +/- 62.8); however, a complete blockade was observed after GHRP-6 (97.3 +/- 7.9), significantly (P < 0.05) lower than after GHRH as well as lower than the GHRP-6-induced GH release in control subjects (P < 0.01). After GHRH plus GHRP-6, the patients with hypothalamopituitary disconnection showed severely reduced secretion (745.3 +/- 67.6; P < 0.01 vs. controls), a value that was not significantly different from the arithmetic addition of levels produced by both compounds administered separately.(ABSTRACT TRUNCATED AT 400 WORDS).
Studies below highlighted red:
Reference:
As biology and the art of science blast through new discoveries and breakthroughs at a pace never seen before, NEW HRT/TRT and other hormone therapeutic protocols are being conducted and shining while delivering results that are making scientist eager to press forward for more data..The truth is science is now truly just grasping an understanding while considering greater possibilities, capabilities with astonishing data..Science knows more about the cosmos than they do with hormones, we're merely scratching the surface and there's always newer data/clinical studies out-dating an others contradicting each other and so on, but its only getting better and more advanced citing new data that is capturing the attention of scientist world wide..
A Few years back I decided to utilize the poor mans HGH option, known as peptides.. The results were not only surprising, but the blood work was stellar when it concerned my IGF-1 levels, surpassing all best serum scores I personally have ever seen or had..
My protocol wasn't HGH, It was CJC dac and MK677 & GHRP-6, scoring low 600's..
As we often see people attempting to re-engineer the wheel, with reports of "the latest new trend" that is spouted through bro-science, these fads seem to come in with blinding speeds, and often fade away soon after..
However, there's some real truth behind some of these findings citing new data never seen before, and these can be supported with actual studies that are currently taken place world wide, so keep on with the personal science experiments, as we are truly our very own science experiment..
So, Let's take a quantitative look at the multitudinous benefits that peptides may actually posses!!!
In the meantime Stay well,
Vision
__________________________________________________ __________________________________________________ __________________
Prolonged Stimulation of Growth Hormone (GH) and Insulin-Like Growth Factor I Secretion by CJC-1295, a Long-Acting Analog of GH-Releasing Hormone, in Healthy Adults
Sam L. Teichman Ann Neale Betty Lawrence Catherine Gagnon Jean-Paul Castaigne Lawrence A. Frohman
The Journal of Clinical Endocrinology & Metabolism, Volume 91, Issue 3, 1 March 2006, Pages 799–805, https://doi.org/10.1210/jc.2005-1536 Published: 01 March 2006
Abstract
Context: Therapeutic use of GHRH to enhance GH secretion is limited by its short duration of action.
Objective: The objective of this study was to examine the pharmacokinetic profile, pharmacodynamic effects, and safety of CJC-1295, a long-acting GHRH analog.
Design: The study design was two randomized, placebo-controlled, double-blind, ascending dose trials with durations of 28 and 49 d.
Setting: The study was performed at two investigational sites.
Participants: Healthy subjects, ages 21–61 yr, were studied.
Interventions: CJC-1295 or placebo was administered sc in one of four ascending single doses in the first study and in two or three weekly or biweekly doses in the second study.
Main Outcome Measures: The main outcome measures were peak concentrations and area under the curve of GH and IGF-I; standard pharmacokinetic parameters were used for CJC-1295.
Results: After a single injection of CJC-1295, there were dose-dependent increases in mean plasma GH concentrations by 2- to 10-fold for 6 d or more and in mean plasma IGF-I concentrations by 1.5- to 3-fold for 9–11 d. The estimated half-life of CJC-1295 was 5.8–8.1 d. After multiple CJC-1295 doses, mean IGF-I levels remained above baseline for up to 28 d. No serious adverse reactions were reported.
Conclusions: Subcutaneous administration of CJC-1295 resulted in sustained, dose-dependent increases in GH and IGF-I levels in healthy adults and was safe and relatively well tolerated, particularly at doses of 30 or 60 μg/kg. There was evidence of a cumulative effect after multiple doses. These data support the potential utility of CJC-1295 as a therapeutic agent.
Topic:
- half-life
- hormone
- adult
- bodily secretions
- insulin-like growth factor i
- plasma
- safety
- somatropin
- multiple-dose regimen
Issue Section:
Endocrine Care
THE USE OF GH for the treatment of children with impaired linear growth has been accepted as an important therapeutic modality for more than 50 yr (1). An unlimited supply of the hormone, made possible by the availability of recombinant GH in the 1980s, permitted expansion of the target population to include GH-deficient adults. Most adults receiving GH today have primary pituitary disease with impaired GH secretory capacity. However, most children being treated with GH have no evidence of pituitary disease and are believed to have an impaired hypothalamic signaling mechanism due to a GHRH neurosecretory dysfunction. GH has also been used for therapy of disorders in children and adults in which pituitary function is either intact or only slightly impaired, such as chronic renal failure and Turner syndrome (in children) and HIV-related wasting and lipodystrophy and burn therapy (in adults).
In patients with intact pituitary function, there has been interest in the use of GHRH rather than GH in the hope of producing a more physiological pattern of tissue exposure to GH than occurs by a single daily injection of the hormone. In fact, several studies in both children and adults have suggested that comparable or near-comparable results can be achieved with GHRH therapy (2–4).
A major limitation in the use of GHRH for therapy, however, is its short half-life. Native GHRH, a 44-amino acid peptide, has a half-life of 7 min (5), which is even shorter than that of GH (12 min) (6), necessitating daily or even more frequent injections. Polyethylene glycol-conjugated GHRH has been studied in an effort to overcome this limitation (7).
A synthetically modified form of GHRH has been linked to a reactive chemical that enables binding to endogenous serum albumin after sc administration. The chemical structure of this compound, drug affinity complex-GH-releasing factor (DAC-GRF; CJC-1295, ConjuChem, Inc., Montréal, Canada) is shown in Fig. 1. The core therapeutic moiety is GHRH-(1–29)NH[SUB]2[/SUB], which contains the full biological activity of GHRH-(1–44)NH[SUB]2[/SUB] modified by substitution of four amino acids that serve to render the compound more resistant to proteolytic cleavage (herein called GRF). GRF is linked by the amino acid, lysine, to a reactive chemical [maleimidoproprionic acid (MPA)] that binds to unpaired thiol (sulfhydryl) groups. The predominant free thiol group available for binding after parenteral administration is the single unpaired cysteine (cysteine 34) in serum albumin. At least 90% of CJC-1295 binds covalently to albumin in this fashion, with trace amounts found bound to fibrinogen and IgG. No other chemical species have been found bound to DAC-GRF after administration (data on file, ConjuChem, Inc.). This binding extends the half-life of the active pharmacophore, resulting in a markedly prolonged duration of action in several animal species (8). Moreover, studies in both dogs and pigs indicate that physiological GH secretion is maintained, and IGF-I levels are enhanced for several days after a single administration.
We assessed the safety, tolerability, pharmacokinetic profile, and effect of CJC-1295 on circulating concentrations of GH and IGF-I in two randomized, placebo-controlled, double-blind, dose-escalating studies in healthy adult subjects.
Fig. 1.
Chemical structure of the CJC-1295 (DAC-GRF). The core therapeutic moiety is a tetrasubstituted GHRH-(1–29)NH[SUB]2[/SUB]. The substituted amino acids are shown in italics. The linker is lysine, and the reactive chemical is maleimidoproprionic acid that binds covalently to the single unpaired cysteine (cysteine 34) in serum albumin.
Subjects and Methods
The subjects consisted of healthy men and women, ages 21–61 yr, with a body mass index of 30 kg/m[SUP]2[/SUP] or less and IGF-I levels in the normal range for age and gender. Appropriately constituted independent ethics committees reviewed and approved each of the studies, and written informed consent of all subjects was obtained before participation.
Study design
Study 1 was an ascending, randomized, double-blind, placebo-controlled single-dose trial performed at SFBC International, Inc. (Miami, FL). Study 2 was an ascending, randomized, double-blind, placebo-controlled multiple-dose trial performed at Kendle International BV (Utrecht, The Netherlands).
In study 1, four sequential, dose-escalation groups were evaluated. These dose levels were 30 μg/kg (n = 6, five active and one placebo), 60 μg/kg (n = 6, five active and one placebo), 125 μg/kg (n = 6, five active and one placebo), and 250 μg/kg (n = 6, five active and one placebo). An additional cohort of 18 subjects (15 active and three placebo) subsequently received 125 μg/kg.
Serum GH was measured on d 0 at 60, 30, and 15 min before study drug dosing; at 15, 30, and 60 min and 2, 3, 4, 6, 8, 10, 12, and 24 h after dosing; and then every 8 h on d 2–3, then daily on d 4, 5, 6, 7, 9, 11, 14, 21, and 28. Serum IGF-I and CJC-1295 were measured on d 0, 1, 2, 3, 4, 5, 6, 7, 9, 11, 14, 21, and 28.
In study 2, 24 subjects were enrolled in one of four sequential dosing cohorts. Group 1 (n = 6, five active and one placebo) received two injections of 30 μg/kg (d 0 and 14), group 2 (n = 6, five active and one placebo) received two injections of 60 μg/kg (d 0 and 14), group 3 (n = 6, five active and one placebo) received three injections of 30 μg/kg (d 0, 7, and 14), and group 4 (n = 6, five active and one placebo) received three injections of 20 μg/kg (d 0, 7, and 14). Sample collection was similar to that in study 1, with the addition of more frequent pre- and postinjection sampling on d 7 (groups 3 and 4 only) and 14 as well as a final sample collection on d 49 in all subjects.
Serial clinical evaluations (vital signs, adverse events, and physical examination) and laboratory safety assessments (serum chemistry, hematology, and urinalysis) were performed ending on d 28 in study 1 and on d 49 in study 2.
Laboratory methods
GH.
Serum GH was measured by a double antibody RIA (Esoterix Laboratory Services, Inc., Calabasas Hills, CA). The assay sensitivity was 0.3 ng/ml, and the coefficient of variation was 10%.
IGF-I.
Serum IGF-I was measured by a double antibody RIA by Esoterix Laboratory Services, Inc., after ethanol extraction and with the addition of IGF-2 as a blocking agent. The assay sensitivity was 10 ng/ml, and the coefficient of variation was 5.4%. Normal ranges for the assay are age and gender adjusted.
Other hormones.
Serum cortisol, prolactin, TSH, and LH concentrations were measured in patients receiving 60 μg/kg CJC-1295 in study 1 by Esoterix Laboratory Services, Inc.
CJC-1295.
Plasma CJC-1295 concentrations were measured by RIA at PPD Development, LP (Richmond, VA), using a rabbit anti-(tetra-substituted)GRF-(1–29) coupled to keyhole limpet hemocyanin and radioiodinated GRF-(1–29). The antibody exhibited 100% cross-reactivity with albumin-bound DAC of the tetrasubstituted GRF-(1–29). There was no cross-reactivity with native GRF-(1–29), native GRF-(3–29), or the DAC of the fragment 12–29 of the tetrasubstituted GRF. There was 25% cross-reactivity with free (nonalbumin-bound) tetrasubstituted GRF-(1–29). The lower limit of detection was 0.2 nmol/liter, and the mean intra- and interassay coefficients of variation were 5.5% and 8.2%, respectively.
Pharmacokinetic analysis.
Pharmacokinetic parameters [peak plasma concentrations (C[SUB]max[/SUB]), time to peak plasma concentrations (T[SUB]max[/SUB]), and area under the curve (AUC)] of CJC-1295, GH, and IGF-I were calculated from the concentration vs. time values for each patient using a compartment model in the single-dose study (WinNonlin Professional version 4.1, Pharsight Corp., Mountain View CA) and a noncompartmental model in the multiple-dose study (WinNonlin Professional version 4.0.1).
Antibody formation.
A validated immunoradiometric assay was used to determine the presence of antibodies to CJC-1295. The anti-CJC-1295 antibody was raised in rabbits by immunization with a CJC-1295 analog [the tetrasubstituted GRF-(1–29)] to which a cysteine residue was added at position 30 to permit direct conjugation to keyhole limpet hemocyanin to make the molecule more immunogenic. This antibody was also used in the assay for plasma CJC-1295 concentrations. In this immunoradiometric assay, tubes are coated with CJC-1295 bound to inactivated MPA. Test samples or affinity-purified rabbit anti-CJC-1295 antibody controls in human serum were added. After incubation, tubes were washed and [[SUP]125[/SUP]I]protein LA (Sigma-Aldrich, St. Louis, MO) was added. After incubation, tubes were washed again, radioactivity was determined in a γ-counter, and the specific binding of the samples was calculated.
Statistical analysis
Mean and variance estimates were calculated for all pharmacokinetic parameters by dose group. C[SUB]max[/SUB] and AUC to the last sampling time (AUC[SUB]t[/SUB]) were log transformed before analysis, and AUC values were calculated using the linear trapezoidal rule. Differences in GH and IGF-I levels and AUC between groups were compared by ANOVA and/or one-tailed t test; P < 0.05 was considered significant. All statistical analyses were performed using SAS version 8.02 (SAS Institute, Cary, NC).
Because all enrolled subjects received at least one dose of the study drug, all available data are included in analyses of safety, pharmacokinetic, and pharmacodynamic parameters. No effort to estimate missing data was made, with the exception of AUC calculations.
Subjects receiving placebo in all dosing groups in each study were pooled for comparison with groups treated with active drug.
Results
Subject characteristics and disposition
The distribution of subjects in the study groups by age and gender is shown in Table 1. The mean overall age of subjects in the two studies was 49.3 ± 1.1 (±se) yr (range, 21–61 yr), and 44% of subjects were men. Although the dosing cohort groups in both studies were generally similar in mean age, gender, height, weight, and BMI, subjects in group 2 in study 2 were younger (mean age, 33 yr), and 80% were men. Two subjects in study 1 (both randomized to the 125 μg/kg dosing cohort) and one subject in study 2 (in group 2) discontinued the study prematurely. The subjects in study 1 withdrew 5 and 22 d after dosing because of mild injection site reactions. The subject in study 2 withdrew 6 d after experiencing multiple mild adverse effects following a single injection of 30 μg/kg.
TABLE 1.
Study drug doses and regimens with subject information
[TABLE="class: cms_table"]
<tbody>[TR]
[TH="align: center"][/TH]
[TH="align: center"]Total no.[/TH]
[TH="align: center"]Age ± sd[/TH]
[TH="align: center"]No. of men[/TH]
[TH="align: center"]No. of women[/TH]
[TH="align: center"]No. of doses[/TH]
[TH="align: center"]Dosing days[/TH]
[/TR]
[TR]
[TD]Study 1[/TD]
[TD][/TD]
[TD][/TD]
[TD][/TD]
[TD][/TD]
[TD][/TD]
[TD][/TD]
[/TR]
[TR]
[TD] Dose[/TD]
[TD][/TD]
[TD][/TD]
[TD][/TD]
[TD][/TD]
[TD][/TD]
[TD][/TD]
[/TR]
[TR]
[TD] 30 μg/kg[/TD]
[TD]5[/TD]
[TD]55 ± 8[/TD]
[TD]2[/TD]
[TD]3[/TD]
[TD]1[/TD]
[TD][/TD]
[/TR]
[TR]
[TD] 60 μg/kg[/TD]
[TD]5[/TD]
[TD]53 ± 7[/TD]
[TD]2[/TD]
[TD]3[/TD]
[TD]1[/TD]
[TD][/TD]
[/TR]
[TR]
[TD] 125 μg/kg[/TD]
[TD]20[/TD]
[TD]50 ± 6[/TD]
[TD]7[/TD]
[TD]13[/TD]
[TD]1[/TD]
[TD][/TD]
[/TR]
[TR]
[TD] 250 μg/kg[/TD]
[TD]5[/TD]
[TD]53 ± 3[/TD]
[TD]1[/TD]
[TD]4[/TD]
[TD]1[/TD]
[TD][/TD]
[/TR]
[TR]
[TD] Pooled placebo[/TD]
[TD]7[/TD]
[TD]52 ± 7[/TD]
[TD]3[/TD]
[TD]4[/TD]
[TD]1[/TD]
[TD][/TD]
[/TR]
[TR]
[TD]Study 2[/TD]
[TD][/TD]
[TD][/TD]
[TD][/TD]
[TD][/TD]
[TD][/TD]
[TD][/TD]
[/TR]
[TR]
[TD] Dose[/TD]
[TD][/TD]
[TD][/TD]
[TD][/TD]
[TD][/TD]
[TD][/TD]
[TD][/TD]
[/TR]
[TR]
[TD] 20 μg/kg[/TD]
[TD]5[/TD]
[TD]43 ± 14[/TD]
[TD]3[/TD]
[TD]2[/TD]
[TD]3[/TD]
[TD]d 0, 7, 14[/TD]
[/TR]
[TR]
[TD] 30 μg/kg[/TD]
[TD]5[/TD]
[TD]33 ± 15[/TD]
[TD]4[/TD]
[TD]1[/TD]
[TD]3[/TD]
[TD]d 0, 7, 14[/TD]
[/TR]
[TR]
[TD] 30 μg/kg[/TD]
[TD]5[/TD]
[TD]40 ± 11[/TD]
[TD]3[/TD]
[TD]2[/TD]
[TD]2[/TD]
[TD]d 0, 14[/TD]
[/TR]
[TR]
[TD] 60 μg/kg[/TD]
[TD]5[/TD]
[TD]57 ± 1[/TD]
[TD]2[/TD]
[TD]3[/TD]
[TD]2[/TD]
[TD]d 0, 14[/TD]
[/TR]
[TR]
[TD] Pooled placebo[/TD]
[TD]4[/TD]
[TD]51 ± 13[/TD]
[TD]2[/TD]
[TD]2[/TD]
[TD]2 or 3[/TD]
[TD]d 0, 14, or d 0, 7, 14[/TD]
[/TR]
</tbody>[/TABLE]
View Large
Plasma CJC-1295 concentrations
In study 1, the mean C[SUB]max[/SUB] of CJC-1295 increased with dose, with values of 2.17, 5.19, 8.16, and17.1 nmol/liter in the 30, 60, 125, and 250 μg/kg single-dose groups, respectively (Fig. 2). Maximum drug concentrations were reached between 1 and 1.5 h in all groups. The mean AUC[SUB]t[/SUB] in the 30, 60, 125, and 250 μg/kg dose groups were 143, 355, 669, and 1276 h·nmol/liter, respectively, with dose-proportionality observed in the 60, 125, and 250 μg/kg dose groups. Terminal elimination rate, systemic clearance, and volume of distribution gradually increased with dose. The mean half-life ranged from 5.8–8.1 d; mean systemic clearances were 0.04, 0.04, 0.05, and 0.05 liters/h/kg, and mean volumes of distribution were 8.1, 9.7, 11.6, and 13.8 liter/kg in the four dose groups, respectively.
Fig. 2.
Plasma disappearance curves of CJC-1295 after a single sc injection. Shown are the mean ± sd half-life. Drug concentrations were generally measurable for at least 12–14 d after injection.
In study 2, maximum CJC-1295 plasma concentrations were 11–32% higher after the injection on d 7 than on d 0 in the two groups that received weekly injections (data not shown). After the d 14 injection, maximum CJC-1295 concentrations were 29–70% higher than those on d 0 in all four groups. Similar increases occurred in AUC[SUB]0–24 h[/SUB] on d 7 (12% and 15%) in groups 3 and 4 and in all four dosing groups on d 14 (31–57%). The 0–24 h AUC values were dose dependent.
Maximum drug concentrations were typically reached within 0.5–2.0 h after injection, but there was a high degree of individual variability. The variability increased with subsequent doses, but did not appear to be dose dependent. Drug concentrations were measurable for 10–14 d. In subjects in whom samples were obtained for up to 14 d, the mean estimated half-life of CJC-1295 ranged from 5.4–9.2 d, and the mean clearance was between 1.1 and 3.3 liters/h. The pharmacokinetic parameters were independent of body weight.
Serum GH concentrations
In study 1, mean preinjection GH concentrations ranged from 0.7–1.1 ng/ml. Mean GH concentrations increased by 2- to 10-fold after single-dose injection of CJC-1295 through d 6 (Fig. 3A). In contrast, mean GH concentrations remained stable in the placebo group. The mean GH AUC[SUB]0–7 d[/SUB] values were elevated in a dose-dependent manner, although only the groups receiving 60, 125, and 250 μg/kg had significant increases compared with the placebo group (Fig. 3B).
Fig. 3.
GH responses to a single sc injection of CJC-1295. A, Serum GH concentrations (mean ± sd) are shown and suggest that pulsatile hormone secretion is maintained. B, Mean GH AUC[SUB]0–7 d[/SUB], expressed as a percent increase over placebo. *, P < 0.05 vs. placebo. Shown are the mean ± sd. Mean maximum concentrations of GH were 6.6, 9.6, 9.9, and 13.3 ng/ml in the 30, 60, 125, and 250 μg/kg groups; mean AUC were 758, 969, 977, and 1370 ng/ml·h, respectively.
The median peak GH level occurred within 1 h in all dosing groups in both studies, with the exception of the group receiving a single 250 μg/kg dose of CJC-1295, in which the median peak GH level occurred at 4 h. The mean peak GH levels were more variable and occurred 0.5–4 h after dosing. The variability was neither dose dependent nor progressive.
Serum IGF-I concentrations
Mean preinjection IGF-I concentrations ranged from 123–152 ng/ml in study 1. IGF-I levels remained elevated compared with baseline for 9–11 d after a single injection of CJC-1295 in all dosing groups in study 1, and mean levels increased by 0.5- to 3-fold over baseline (Fig. 4A). Mean IGF-I AUC[SUB]0–7 d[/SUB] values were elevated in a dose-dependent manner, reaching statistical significance compared with baseline in the groups receiving 60, 125, and 250 μg/kg (Fig. 4B). IGF-I levels exceeded the age- and gender-adjusted normal ranges only in subjects receiving 250 μg/kg CJC-1295. In contrast, mean IGF-I levels in the placebo group remained relatively stable during the same period. The time to peak IGF-I levels was dose dependent, occurring 2–3 d after administration of the lowest three doses, but not until 4 d after the highest dose. IGF-I levels remained at a plateau for up to 7 d, after which they gradually declined toward baseline. IGF-I levels remained elevated for at least 2 wk after injection in patients receiving the two highest doses.
Fig. 4.
IGF-I responses to a single sc injection of CJC-1295. A, Serum IGF-I concentrations (mean ± sd) are shown. B, Mean IGF-I AUC[SUB]0–7 d[/SUB], expressed as a percent increase over placebo. *, P < 0.05 vs. placebo. Shown are the mean ± sd. The shaded area marked is the upper limit of normal (U.L.N.) for age- and gender-matched cohorts. Mean maximum concentrations of IGF-I were 232, 319, 328, and 435 ng/ml in the 30, 60, 125, and 250 μg/kg groups; mean AUC were 91, 127, 119, and 172 μg/ml·h, respectively.
In study 2, mean IGF-I levels increased within 8 h of CJC-1295 injection and remained above baseline levels through d 28 (Fig. 5). Mean IGF-I values remained elevated above baseline before the second and/or third doses in all CJC-1295-treated groups, although this reached significance only for group 4 (P < 0.03 for group 4 and P < 0.07 for group 3, by paired t test). Maximum IGF-I levels after the second and/or third injections were progressively greater than after the initial injection (data not shown). In addition, the T[SUB]max[/SUB] for IGF-I was progressively shorter after subsequent injections (data not shown).
Fig. 5.
IGF-I responses to multiple sc injections of CJC-1295. Serum IGF-I concentrations (mean ± se) are shown. Arrows indicate days of injection. A, Changes in serum IGF-I levels after three weekly injections of CJC-1295 or placebo. B, Changes in serum IGF-I levels after two biweekly injections of CJC-1295 or placebo. In both A and B, for d 0–7 and 14–21, the entire pooled placebo group (n = 4) is plotted, because all these subjects received placebo injections on d 0 and 14. In A, for the period from d 7–14, only the two subjects who received placebo injections on d 7 are plotted (i.e. three weekly injections). In B, for the period from d 7–14, only the two subjects who received placebo injections on d 0 and 14 are plotted (i.e. two biweekly injections).
Mean AUC[SUB]0–7 d[/SUB] and AUC[SUB]0–14 d[/SUB] for IGF-I were significantly increased in group 2 (60 μg/kg) compared with group 1 (30 μg/kg; P = 0.041 and P = 0.043, respectively) and were significantly greater in both groups compared with the placebo group (P = 0.003 and P = 0.005, respectively). Mean AUC[SUB]0–7 d[/SUB], AUC[SUB]7–14 d[/SUB], and AUC[SUB]14–21 d[/SUB] were all significantly higher in group 3 (30 μg/kg) than in group 4 (20 μg/kg; P = 0.010, P = 0.020, and P = 0.026, respectively).
Other than age, no significant predictors of IGF-I or GH response, including gender, baseline weight or body mass index, and lipid or glucose levels, were observed.
Other hormonal effects
There were no significant increases in serum cortisol, prolactin, TSH, or LH levels after a single injection of CJC-1295 (60 μg/kg), thus confirming the specificity of CJC-1295 for GH secretion (data not shown).
Safety
In the single-dose study, adverse events were reported in 33 (94%) vs. two (29%) subjects in the active and placebo groups, respectively; all were of mild to moderate severity, and none required medical intervention to resolve. Injection site reactions (irritation, erythema, induration, pain, or itching) occurred transiently (up to several hours) in approximately 70% of subjects receiving CJC-1295 and rarely in subjects receiving placebo. Injection site reactions tended to be more severe and/or prolonged after higher doses, with residual induration lasting up to 5 d. No local reaction exceeded 10 cm in diameter, and all resolved spontaneously. Transient urticarial rashes at the injection site occurred in almost 30% of subjects and were not dose related. Other adverse events reported in actively treated subjects included headache (63%), diarrhea (43%), and systemic vasodilatory reactions (flushing, warmth, and transient hypotension; 30%); all were more common at higher doses (125 or 250 μg/kg). Headache and diarrhea occurred occasionally at various times during the 7 d after dosing. Of these adverse events, only headache occurred in the placebo-treated group (14%). Overall, the adverse events observed at 250 μg/kg were moderate in severity and resolved spontaneously after a few hours, whereas the 125 μg/kg dose of CJC-1295 was considered well tolerated.
Injection site reactions (irritation, erythema, induration, pain, or itching) were reported in all actively treated subjects in study 2; all were mild in severity. Reactions were up to 10 cm in diameter. Mild injection site erythema (<2–3 cm) was reported in three of four placebo-treated subjects (75%) as well as induration and urticaria (<1–2 cm; 25%). Flushing occurred only in actively treated subjects, occurred within 30 min of injection, and resolved within 1–2 h. The incidence of flushing was dose dependent, with an incidence of 40% after low-dose and 100% after high-dose injections.
Other adverse events included transient loose stools/diarrhea (45% and 100% incidence in the 125 and 250 μg/kg groups, respectively), headache (non-dose dependent and ranging from 20–80% depending on the dose group), and nausea or abdominal pain (20%). Of these adverse events, only headache occurred in the placebo-treated group (14% in study 1 and 50% in study 2).
The incidence and severity of all adverse events were gender independent. There were no consistent changes in blood or urine laboratory values, including glucose levels and liver function studies, or in electrocardiographic findings in either study. No significant antibody formation was detected in subjects who received the active study drug.
A single subject in study 2 reported mild, transient (resolving fully and spontaneously in <24 h) involuntary leg muscle contractions and some loss of coordination after receiving the second biweekly injection of 30 μg/kg. Two subjects experienced transient dizziness and hypotension after the first injection of 30 μg/kg that resolved spontaneously and did not recur after subsequent injections of CJC-1295.
Discussion
This report describes the safety, pharmacokinetic profile, and pharmacodynamic effects of CJC-1295, a synthetic analog of GHRH that permanently and covalently binds to serum albumin after administration. Results of the single-dose and multiple-dose studies demonstrate a prolonged half-life of CJC-1295 (∼6–8 d) after sc administration, with measurable drug concentrations for 10–13 d after single or multiple doses. In addition, there was clear evidence of a dose-responsive and sustained biological effect, with elevated GH and IGF-I serum concentrations persisting for at least 6 and 14 d, respectively, after single doses of CJC-1295. In the multiple-dose study, there was a cumulative effect after two or three injections of CJC-1295 administered weekly or biweekly, with elevated levels of both GH and IGF-I above baseline on d 14 in most subjects. CJC-1295 was safe and generally well tolerated, particularly at doses of 30 and 60 μg/kg.
Treatment with human GH typically consists of a single daily injection of the hormone, resulting in transient supraphysiological levels, followed by a decline to baseline. However, failure to mimic the physiological pulsatile nature of GH secretion may preclude optimal therapeutic effects and may contribute to some of the adverse effects that have been observed even in the presence of normal serum IGF-I levels. In contrast, injections or infusions of GHRH stimulate the pulsatile release of GH (9, 10), but the short plasma half-life (7 min) (5) renders GHRH impractical for therapeutic use. Therefore, the availability of a GHRH preparation with sustained effect has important therapeutic potential.
The half-life of CJC-1295, as predicted from preclinical animal studies (8), was substantially prolonged compared with that of native GHRH, ranging from 5.8–8.1 d in the single-dose study and from 5.4–9.2 d in the multiple-dose study. Maximum concentrations were typically reached within 2 h after injection and exhibited a slow exponential decrease over several days. The disappearance rates were not dose dependent, although serum CJC-1295 concentrations were proportional to the dose injected. In the multiple-dose study, C[SUB]max[/SUB] and AUC[SUB]0–24 h[/SUB] were 17% greater on d 7 than on d 0 and from 30–70% greater on d 14 than on d 0.
Administration of single doses of CJC-1295 resulted in a 2- to 10-fold increase in mean serum GH levels in all dosing groups, which was dose incremental and persisted for up to 6 d. Similarly, a dose-related increase in mean serum IGF-I levels was observed at all dose levels, ranging from 1.5- to 3-fold and persisting for up to 14 d. Administration of ascending multiple doses of CJC-1295 resulted in elevated levels of GH, similar to those observed after a single dose.
In contrast, elevations in IGF-I levels showed a progressive effect over time, particularly in subjects receiving CJC-1295 every 7 d. Results of the multiple-dose study suggest both a cumulative pharmacokinetic effect [i.e. persistence of elevated predose levels of IGF-I in all dosing groups except group 1 (i.e. two injections of 30 μg/kg)] and a pituitary priming effect (i.e. progressively greater C[SUB]max[/SUB] and progressively shorter T[SUB]max[/SUB] after serial dosing). The data indicate that a minimum dosing interval of 7 d appears reasonable. The most appropriate dosing interval will be determined based on actual efficacy and safety data from longer-term therapeutic studies in patients with various clinical conditions.
No serious adverse reactions were reported in either study. The most frequently reported adverse events in subjects receiving CJC-1295 were injection site reactions, consisting of transient pain, swelling, and induration that were sometimes accompanied by local urticaria. Injection site reactions tended to be more severe and/or prolonged at higher dose levels. Headache, diarrhea, and flushing were also observed, with occasional transient and mild hypotension, but occurred primarily at higher doses.
Adverse effects complicate the use of GH in the treatment of HIV-associated metabolic conditions such as wasting and lipodystrophy. Although increases in the daily dose of GH from 1 to 6 mg are associated with dose-responsive benefits (11–13), doses of 2–3 mg/d or greater are associated with edema, arthralgias, and glucose intolerance. These side effects can become dose limiting. In the current studies, none of the subjects experienced these adverse effects. Future clinical trials on this disorder will confirm whether the use of GHRH rather than GH will circumvent these problems, as has been suggested in recent publications (4, 14).
In summary, a single sc administration of CJC-1295 produced sustained elevations of serum GH and IGF-I levels in normal subjects for nearly 2 wk. Weekly or biweekly administration of CJC-1295 resulted in stimulation of GH and IGF-I secretion for at least 7 d. Both single and multiple doses of CJC-1295 over 2 wk were safe and generally well tolerated, particularly at doses of 30 and 60 μg/kg. Future studies are indicated to evaluate the clinical utility of treatment with CJC-1295 in patients with intact GH secretory capacity.
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Last but not least
Just some more information about CJC-1295; it's worth the time invested while reading..I've been saying for sometime now that peptides are the way to go.. For GHRP-6 it's not for the faint-hearted, if you struggle with BS stability I would not suggest it although a lot of peptides may give a "false sense" of going hypo (LBG), it mainly comes with the territory of GHRP-2/6..Although it's primarily a false sense it can feel and almost demonstrate just as equal or if not more server than a real instances of going hypo by ones own diet/exercise and or genetics and so on..Having carbs/food on hand can easily mitigate these sides for those that are sensitive, furthermore it can be capitalized on and taken advantage of for those that have a tendency to not have the urge or desire to eat, this onset may provide the ideal platform for having the need or desire to want to consume food, almost instantaneously for some..
The combo of the both have made rest and recovery a beautiful thing in respect to feeling sleepy and wanting to shut my eyes almost anywhere at any given time, not to the point where it hinders my daily life but if I wish to nap, I can, and I will wherever I wish and even a small nap at 10-20 mins I wake up feeling as if I was out for hrs, groggy, but rested and ready to pounce!
Scientific research CJC-1295
In the experiment of Ionescu M. et al. [5], men aged 20-40 years were given a single dose of CJC-1295 (60 or 90 microg / kg body weight CJC-1295), a blood sample was collected every 20 minutes. This means that for a person weighing 90 kg, 5400 mcg (5.4 mg) or 8100 mcg (8.1 mg) CJC-1295 was administered.
Results? After a single dose of CJC-1295:
- a 7.5-fold increase in baseline growth hormone was noted,
- total secretion of growth hormone increased by 46%, while the level of IGF-1 increased by 45%.
In another study, Teichman SL. Both co-workers [1] lasted 28 or 49 days. Healthy people aged 21-61 participated in it.
After a single injection of CJC-1295 (30 or 60 microg / kg body weight CJC-1295), there were:
- average 2-10 fold increase in plasma growth hormone for 6 days,
- in turn, mean plasma IGF-1 concentrations increased from 1.5 to 3 times for a period of 9-11 days,
- the half-life CJC-1295 is 5.8-8.1 days,
- after several injections of CJC-1295, the level of IGF-1 was increased above baseline values to 28 days.
GHRH agents interact through other mechanisms and synergistically together with GHRP, causing a much stronger GH and IGF-1 burst.
This is clearly demonstrated by numerous scientific studies, including experiment carried out by Popovic V. et al. [2].
One of the studies reported:
- GHRH - growth hormone releasing hormone (100 mcg, intravenously),
- GHRP-6 (90 mcg, intravenously),
- GHRH (growth hormone releasing hormone) + GHRP-6.
What was the effect on growth hormone?
- After growth hormone releasing hormone (GHRH), an increase in the amount of GH to the level of 483.7 +/- 99.2,
- After GHRP-6 up to level 1434.8 +/- 393.0,
- After GHRH (growth hormone releasing hormone) + GHRP-6 up to level 3 771,5 +/- 399.6!
This study clearly shows that growth hormone releasing peptides should not be used alone, without the administration of, for example, GHRH analogs (tesamorelin, TH9507, CJC-1295, MOD GRF 1-29, MR-356, MR-361, JI-38, MR-356 403, MR-367).
Side effects of using CJC-1295:
Unfortunately, there is no information on this subject, it can be suspected that, like tesamorelin CJC-1295, it can trigger:
- local inflammatory reactions at the injection site (erythema, pruritus, irritation, edema),
- rarely diabetes (!), the antagonistic effect of growth hormone on insulin!
- hyperglycemia,
- muscle aches,
- muscle stiffness,
- paresthesia,
- night sweating,
- rash.
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Study below:
Prolonged stimulation of growth hormone (GH) and insulin-like growth factor I secretion by CJC-1295, a long-acting analog of GH-releasing hormone, in healthy adults.
Teichman SL1, Neale A, Lawrence B, Gagnon C, Castaigne JP, Frohman LA.
Author information
Abstract
CONTEXT:
Therapeutic use of GHRH to enhance GH secretion is limited by its short duration of action.
OBJECTIVE:
The objective of this study was to examine the pharmacokinetic profile, pharmacodynamic effects, and safety of CJC-1295, a long-acting GHRH analog.
DESIGN:
The study design was two randomized, placebo-controlled, double-blind, ascending dose trials with durations of 28 and 49 d.
SETTING:
The study was performed at two investigational sites.
PARTICIPANTS:
Healthy subjects, ages 21-61 yr, were studied.
INTERVENTIONS:
CJC-1295 or placebo was administered sc in one of four ascending single doses in the first study and in two or three weekly or biweekly doses in the second study.
MAIN OUTCOME MEASURES:
The main outcome measures were peak concentrations and area under the curve of GH and IGF-I; standard pharmacokinetic parameters were used for CJC-1295.
RESULTS:
After a single injection of CJC-1295, there were dose-dependent increases in mean plasma GH concentrations by 2- to 10-fold for 6 d or more and in mean plasma IGF-I concentrations by 1.5- to 3-fold for 9-11 d. The estimated half-life of CJC-1295 was 5.8-8.1 d. After multiple CJC-1295 doses, mean IGF-I levels remained above baseline for up to 28 d. No serious adverse reactions were reported.
CONCLUSIONS:
Subcutaneous administration of CJC-1295 resulted in sustained, dose-dependent increases in GH and IGF-I levels in healthy adults and was safe and relatively well tolerated, particularly at doses of 30 or 60 microg/kg. There was evidence of a cumulative effect after multiple doses. These data support the potential utility of CJC-1295 as a therapeutic agent.
Study Below:
Blocked growth hormone-releasing peptide (GHRP-6)-induced GH secretion and absence of the synergic action of GHRP-6 plus GH-releasing hormone in patients with hypothalamopituitary disconnection: evidence that GHRP-6 main action is exerted at the hypothalamic level.
Popovic V1, Damjanovic S, Micic D, Djurovic M, Dieguez C, Casanueva FF.
Author information
Abstract
GH-releasing peptide (GHRP-6; His-D Trp-Ala-Trp-D Phe-Lys-NH2) is a synthetic compound that releases GH in a specific and dose-related manner through mechanisms and a point of action that are mostly unknown but different from those of GHRH. In man, GHRP-6 is more efficacious than GHRH, and a striking synergistic action on GH release is observed when GHRP-6 and GHRH are administered simultaneously. Based on such a synergistic action, it has been hypothesized that GHRP-6 acts through a double mechanism by actions exerted both at the pituitary and hypothalamic levels. The aim of the present study was 2-fold: 1) to further characterize the mechanism of action and synergistic effects of GHRP-6; and 2) to study its action in patients with hypothalamopituitary disconnection. Twelve patients with different neuroendocrine pathologies leading to a state of hypothalamopituitary disconnection (functional stalk section) and 11 age- and sex-matched normal controls were studied. Each subject underwent 3 tests on separate occasions, being challenged with GHRH (100 micrograms, i.v.), GHRP-6 (90 micrograms, i.v.), or GHRH plus GHRP-6. GH was analyzed as the area under the curve (mean +/- SE, micrograms per L/120 min). In normal subjects GH secretion was 483.7 +/- 99.2 after GHRH, 1434.8 +/- 393.0 after GHRP-6, and 3771.5 +/- 399.6 after GHRH plus GHRP-6; the level of GH secreted after GHRH plus GHRP-6 treatment was significantly (P < 0.05) higher than after the arithmetic sum of GH levels after both compounds administered separately. In the group of patients with hypothalamopituitary disconnection, the level of GH secreted after GHRH was similar to that in controls (423.4 +/- 62.8); however, a complete blockade was observed after GHRP-6 (97.3 +/- 7.9), significantly (P < 0.05) lower than after GHRH as well as lower than the GHRP-6-induced GH release in control subjects (P < 0.01). After GHRH plus GHRP-6, the patients with hypothalamopituitary disconnection showed severely reduced secretion (745.3 +/- 67.6; P < 0.01 vs. controls), a value that was not significantly different from the arithmetic addition of levels produced by both compounds administered separately.(ABSTRACT TRUNCATED AT 400 WORDS).
Studies below highlighted red:
Reference:
- Teichman SL1, Neale A, Lawrence B, Gagnon C, Castaigne JP, Frohman LA. "Prolonged stimulation of growth hormone (GH) and insulin-like growth factor I secretion by CJC-1295, and long-acting analogue of GH-releasing hormone, in healthy adults". J Clin Endocrinol Metab. 2006 Mar; 91 (3): 799-805. Epub 2005 Dec 13. https://www.ncbi.nlm.nih.gov/pubmed/16352683
- Popovic V1, Damjanovic S, Micic D, Djurovic M, Dieguez C, Casanueva FF. "Blocked growth hormone-releasing peptide (GHRP-6) -induced GH-releasing hormone in patients with hypothalamopituitary disconnection: GHRP-6 main action is exerted at the hypothalamic level ". J Clin Endocrinol Metab. 1995 Mar; 80 (3): 942-7. https://www.ncbi.nlm.nih.gov/pubmed/7883854
- Xiangyang Xia, 1 Quanwei Tao, 2 Qunchao Ma, 3, 4 Huiqiang Chen, 5 Jian'an Wang, 3, 4 and Hong Yu Growth "Hormone-Releasing Hormone and Its Analogues: Significance for MSCs-Mediated Angiogenesis" https: // www.ncbi.nlm.nih.gov/pmc/articles/PMC5059609/
- Knoop A1, Thomas A1, Fichant E2, Delahaut P2, Schänzer W1, Thevis M3,4. "Qualitative identification of growth hormone-releasing hormones in human plasma by means of immunoaffinity purification and LC-HRMS / MS." https://www.ncbi.nlm.nih.gov/pubmed/26879649
- Ionescu M1, Frohman LA. "Pulsatile secretion of growth hormone (GH) persisted in continuous stimulation by CJC-1295, and long-acting GH-releasing hormone analog." https://www.ncbi.nlm.nih.gov/pubmed/17018654 .
