Best filtration system for bigger volumes

[bonacris]

new to this forum and just joined because of the activity in the Hb section. great site and hope to contribute as best I can.

does anyone use a Buchner funnel and Buchner flask? using sterile filter discs it would surely cut down the cost of bottle top filters.

what is the best filtration system. the syringe filters are good for a test sample but not for larger volumes and I don't like the idea of ordering loads of bottle top filters

 

[Doctor9]

The buchner funnel will make a good *pre* filter - in fact it will be quite efficient - however, don't try to sterilize with it unless you have a good way of sterilizing the funnel, the flask and the filter disks.

That final 0.2 micron filtration is killer - no point running it through a sterile filter into a non-sterile bottle, sadly.

THey do make glass filtration systems that are easier to sterilize that buchner funnels that use membrane filters, but you do still have to be sure everything is sterile, or you're no further ahead.

Bottle top filters aren't cheap, admittedly, but *if* you do a proper pre-filtration, you can get quite a lot of volume through them, bringing the cost per unit of product down.

Doctor9

 

[bonacris]

The buchner funnel will make a good *pre* filter - in fact it will be quite efficient - however, don't try to sterilize with it unless you have a good way of sterilizing the funnel, the flask and the filter disks.

That final 0.2 micron filtration is killer - no point running it through a sterile filter into a non-sterile bottle, sadly.

THey do make glass filtration systems that are easier to sterilize that buchner funnels that use membrane filters, but you do still have to be sure everything is sterile, or you're no further ahead.

Bottle top filters aren't cheap, admittedly, but *if* you do a proper pre-filtration, you can get quite a lot of volume through them, bringing the cost per unit of product down.

Doctor9

Thank you. Do you have a link to these glass filtration systems or do you put it together yourself from various places.

Thanks again

 

[Presser]

welcome to MC brutha

 

[bonacris]

welcome to MC brutha

Thank you Presser.

 

[Doctor9]

This is one possible supplier - you also need to get the filter membranes themselves. You need a source of vacuum as well, either a vacuum pump or a water aspirator.

http://www.membrane-solutions.com/glass_solvent_filter_unit.htm

they also have the membranes, but only in large quantities (box of 400) - other online suppliers would likely have smaller quantities available. (I recommend PTFE as the most inert material with a PP backing, if necessary)

Doctor9

Doctor9

 

[beeflover]

Welcome brother.
You won't be disappointed joining this forum.
Some great peeps hear wit good home brew exp.
mi joy your stay.

 

[BEASTZ6]

Welcome to MC. Best home brew sub-forum on the net.

 

[bonacris]

This is one possible supplier - you also need to get the filter membranes themselves. You need a source of vacuum as well, either a vacuum pump or a water aspirator.

http://www.membrane-solutions.com/glass_solvent_filter_unit.htm

they also have the membranes, but only in large quantities (box of 400) - other online suppliers would likely have smaller quantities available. (I recommend PTFE as the most inert material with a PP backing, if necessary)

Doctor9

Doctor9

I ordered the glass filter holder, clamp and all the top part. along with a few different sterile media bottles so I can do a few batches in one sitting but I cant find sterile membrane filter paper.

is there such a thing or do I need to sterilize it myself. sterile nylon or PTFE would suit me best

- - - Updated - - -

Thanks for the welcome BEAST and Beeflover. love the forum

 

[Userat204]

Imo if you want to keep it simple and want a good quality filter that you can use even with a hand pump and crank out a few 1000ml. The what man zapcap is hard to beat. Add a prefilter which it comes with one, but I also run about 15ml of BA thru it before I ever use it. and I mean make sure to pull out every drop you can. You Gdansk set these to about 20 or 25 on a hand pump and kerb them go. They are made out of a flexible material so they don't crack and leak like most filters will

the only reason I do this is BA is a bacteriostat. It will prevent the new growth of bacteria. The purpose of the filter paper is to catch the bacteria and all the garbage includinging viruses in a .2 the thought Is once warm oil containing bacteria passes into th filtration, it's going to start growing exponentially rhendering the filter isles in a short time. By doing a pre run with a little BA yea you may get a little extra in 400 to 1000 ml of gear but not enough that it's everr bothered me or anyone who has used it but it may just make your filter go a little faster for a little longer because it doesn't have bacteria growing once you stop for a minute. What man states these can be reused in the same setting as long as the filter stays wet. I wouldn't necessarily go switching compounds all though you could and I would save them and use them for multiple days. what they mean buy 're use is in the same time you are heating more and still on your media bottle or even switching bottles and keeping the paper wet. I always add a new prefilter paper y2k take out anything large which also preserves the filter for most use. But in one setting of use I toss it when done for the day but I will have multiple going at one time.

If you have a few hundred extra to spend Irecommend getting a laboratory pump,medical pump or perlistatic pump. They are quiet, and all you have to do is set the vaccum pressure and in this case I would use a NALGENE FAST CAP, this thing you can run insane amounts thru and they come in size .1 which has the added benefit of filtering out microbes. I bought a pump brand new on eBay for 329 there are no moving parts so no oils or maintenance and it works like a champ. I'm talking liters or gallons per hour. Which is a bit more than I need but I wanted the fast caps and the .1 filters

But for your everyday set up I don't think you can go wrong with a whatmam zapcap and a 77mm prefilter paper. These are great for guys just getting into bottle tops. I used a lot of other brands with a lot of frustration. They didn't want to filter what they claimed and would crack or just start to leak. You won't run into that issue with a zap cap. they are essentially dummy proof.

 

[6-fingers]

I am not going to discount what others have said, as I do not know how much you are trying to filter nor how quickly. I used bottle top once, about a decade ago, and it was nightmare. Personally, as soon as individual is ready to graduate from syringe filters, I feel they should go directly to peristaltic pump and whatman polycap filter.

For pump, get this one:http://www.novatech-usa.com/Products/Dolphin-Peristaltic-Pumps-Parts/UD75-XA-LSAUXXX

UD75-XA-LSAUXXX
MEC-O-MATIC Dolphin Series Peristaltic Metering Pump
97 GPD @ 25 PSI, 115 VAC
(Manual Run Time Control)

If you only purchase one, it will cost you $240.37.
The first time you use it, you will know you got your money's worth.

Used in conjunction with Whatman Polycap 75AS, 0.2um, with Filling Bell, you can filter approximately 255ml/min. Or 1 Liter of media every 4 minutes.

I recommend using an autoclaved 2000ml Hybex media bottle.

Once you are proficient, you will be able to throw Polycap onto Hybex bottle and start up pump, then occupy yourself with other lab tasks for entire 8 minutes it takes to filter 2L of media into Hybex bottle, stop pump and cap bottle, move filter onto another Hybex and crank it up again.
Once all of your media has been filtered, use a bottle top dispenser to fill vials. Even manual dispenser and you should be able to run 50-75/min.

 

[Userat204]

6 fingers is dead on. But if your talking 400 to 500ml I would simply use a whatman zap cap. For me thew are the only bottle tops worth using. Every other one I tried would crack or begin to leak at the filter like leak out the sides and that was at any amount of vacuum but with a zapcap they use a more flexible plastic so even if u were to use a cheap hand pump you can have it done in 15 minutes deoendingb on the hormone. But for any real volume 6 is dead on andv good looking out on a link. I found mine on eBay for 300. It's the best thing I've ever used. I will still use a syringe filer with tne, t suspension and win oil and water based as well as inject dbol and drol its just personal preference on those for me. I literally will filter 1-2ml into my bac water then shake and repeat it's time consuming but my recipe is excellent and all painless and I'm not doing that type of volumrb these days. If I do 1000ml in a day anymore I'm good but I may do that with a handful of hormones and I'm good for a few weeks to a coupe months at times. So again 6 is correct 100%, but fircmuch smaller set ups zapcaps are imo the best bottle top. Just my 2¢.

 

[Giru]

Hello

This post is interesting for me. Soon i have to do a brew around 600 to 700 ml of different compounds(susta, bolde, tren, masteron and prop). I am looking to do it with a bottle top filter of course, but i was thinkin about millipore, but after read this post you change my mind. I think i am gonna use a whatmam zapcap as Userat204 sais, but i have some question...
How much pressure can i apply to that top bottle filters?
If for example i filter 100m susta, can i filter after, maybe 30 min after(the time that it takes to do another solution with the beaker) another brew for ex bolde by the same filter? or should i clean the filter like you said Userat204 with 15ml of BA?
For how many ml can a whatmam zapcap being used?

Thanks!

 

[Userat204]

Giru, BA won't really clean the filter but if you are making multiple compounds you can use the same filter a couple timesc as long as it's wet and preferably in the same session. I run a little BA thru my zap cap when new because it prevents the growth of new bacteriia. If you think about it and this is just my theory your filter catches bacteria among other things but it's a perfect breeding ground for bacteria to grow once in the filter so hopefully the BA will prevent bacterial growth and it won't clog up your filter so quickly.

But on a zap cap I get the pressure up to 25 no problem. If u use a millpore good luck. I couldn't get over 10 on the pressure I can't think of the initials for the pressure measuement right now but those are a hard plastic abd crack very easily. If you want to use a bottle top use a zap cap and I add a pre filter paper to it each time. If you look on medlab site under pumps I believe maybe filters they have a. 70+ mm filter paper that fits well. Use that. Change it each time u start a new batch. But get a few .22 depending on how u plan to make it. I will use the same zap cap for a couple hormones in one sitting but I leave out 20ml of my total oil and run it thru warmed at the end to hopefully draw out all the hormone then I start the next. Its pretty simple really but zap caps are the only bottle tops that ever worked well for me and make sure you have sterile glass media bottles you are filtering into.

 

[Giru]

woooow Userat, amazing explanation everything is clear now. Thanks!!

 

[Pristi1340]

Does anyone bake instead of filtering? 67 mentioned to me that he doesn't filter anymore, pretty interesting to say the least

 

[bonacris]

Does anyone bake instead of filtering? 67 mentioned to me that he doesn't filter anymore, pretty interesting to say the least

Any link to where he says this?

 

[Pristi1340]

Any link to where he says this?

He told me in email Bona

 

[Ohyea]

Once all of your media has been filtered, use a bottle top dispenser to fill vials. Even manual dispenser and you should be able to run 50-75/min.

Where is the best place to find a 45mm bottle dispenser? I can't find one under 575$. Is that just what they run?

 

[bonacris]

He told me in email Bona

Can you PM it to me??? If you don't want to I understand man