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Of the minority of men taking steroids who actually get blood tests, 99% of them get inaccurate assays.Most doctors don’t realize the importance of high sensitivity testing when it comes to men in general, and even more so in steroid users.The difference between high sensitivity testing and what you have probably been getting in the past can be the entire reference range, or in some cases, it may even be detecting metabolites or analogs of other hormones.This is not limited to just testosterone either.Measuring Estradiol via Roche ECLIA (Electrochemiluminescence Immunoassay) instead of liquid chromatography with tandem mass spectrometry (LC/MS-MS) has similar limitations, as do other hormone tests with insufficient analytical sensitivity.[embedded content]Table of ContentsThe Most Accurate Total Testosterone Blood TestThe gold standard for accurately measuring total testosterone levels is high-performance liquid chromatography with tandem mass spectrometry (LC/MS-MS).Recent studies have shown that the current assays used in total testosterone testing lack the required sensitivity to produce accurate results when working with very low concentrations.This can be especially problematic when trying to accurately measure total testosterone levels in severely testosterone deficient men, normal or testosterone deficient women, and children.In addition, these tests have proven via my own personal tests to detect synthetic anabolic androgenic steroids as testosterone.This issue can be corrected by newer methods based on mass spectrometry.The Most Accurate Free Testosterone Blood TestThe gold standard for accurately measuring free testosterone levels is equilibrium dialysis.Equilibrium ultrafiltration is also sufficient.A comparison of results obtained via equilibrium dialysis and equilibrium ultrafiltration found that the two methods correlated closely (r = 0.97) [R].Direct analog enzyme immunoassay (EIA) is inaccurate and has shown in my own personal tests to detect synthetic anabolic androgenic steroids as testosterone.My Blood Test Results Comparing ECLIA And EIA Vs. LC/MS-MS And Equilibrium UltrafiltrationThis is an example that very clearly shows how important high sensitivity testing can be.I went in for a blood test during a Nandrolone-only experiment.I had no Testosterone in my system whatsoever.Expectedly, by assessing my Total Testosterone level via liquid chromatography with tandem mass spectrometry (LC/MS-MS) and my Free Testosterone level via equilibrium ultrafiltration, we can see that my Testosterone levels were crashed.Both the total and the free were lower than a healthy female.This is what you should see in your blood work if you’re on just Nandrolone.The only Testosterone being produced in my body was indirectly via the trace amounts of androgens produced in my adrenal cortex, which is why the value wasn’t completely bottomed out at 0.I’ve mentioned many times the importance of getting high sensitivity testing done for hormone levels and how Nandrolone will register as Testosterone in primitive garbage blood tests.This is another great example of this.In addition to high sensitivity testing, I had the same blood tested using electrochemiluminescence immunoassay (ECLIA) for my Total Testosterone level, and direct analog enzyme immunoassay (EIA) for my Free Testosterone level.These were the test results using the exact same blood sample with the terrible default assays that doctors will use to determine how to treat you, and that labs will give you in the majority of your blood work panels.According to ECLIA and EIA, I had a normal Total Testosterone and Free Testosterone level.This just one example of why getting accurate hormone testing is critical.My Testosterone levels were actually in the gutter, but ECLIA and EIA couldn’t even tell the difference between Testosterone and 19-nortestosterone in my blood.Where To Order Accurate Blood TestsMost labs offer total testosterone (LC/MS-MS), and some offer free testosterone (equilibrium ultrafiltration).However, very few self-pay labs offer free testosterone (equilibrium dialysis).Of the ones that do offer it, it is extremely expensive and it does not come in a package with a total testosterone test.If your doctor will write you a requisition for it, great.If not, just get equilibrium ultrafiltration.Personally, I drive to the US just so I can get LC/MS-MS and equilibrium ultrafiltration testing done.This is the exact test I order every time to accurately check my testosterone levels: Testosterone, Free, Equilibrium Ultrafiltration With Total Testosterone, LC/MS-MSRelated

2020 – NPC Ronnie Coleman Classic Sat Apr 4th Dallas Market Hall, 2200 N Stemmons Fwy, Dallas, TX 75207, USA map    #metroflexevents#metroflexevents-#ronniecolemanclassic-#ronniecoleman-#bodybuilding-#strongman-#powerlifting-#NPC-#IFBB-#Bikini-#physique-#fitness-#grappling-#jiujitsu-#armwrestling-#figure-#fortworthconventioncenter-#fitnessexpo#strongman-#stongwoman-#metroflexplano-#metroflexnorthdallas-#metroflexevents#texasbodybuilding-#npctexas-#texasbodybuildingcontest 2020 NPC Ronnie Coleman Classic NATIONAL QUALIFIER- Sanction# 3293 – April 4th – Dallas Market Hall 2200 N Stemmons Fwy, Dallas, TX 75207

NPC Texas Legends Championships Sat Feb 22nd Round Up Inn, 3400 Burnett Tandy Dr, Fort Worth, TX 76107, USA map  2020 NPC Texas Legends Championships metroflexevents.com NATIONAL QUALIFIER- Sanction # 3863 February 22nd, Round Up Inn-3400 Burnett Tandy Dr. Fort Worth Texas 76017

[embedded content]Contrary to popular belief, more is not better when it comes to exogenous GH use for fat loss.There is a ceiling on lipolysis, and the highest GH dose for maximizing fat loss per administration is much lower than most people think. As you can see here in the following study assessing the pharmacokinetics and acute lipolytic actions of GH, there were significant dose-response effects when comparing the incremental area under the curve of both free fatty acids and blood 3-hydroxy-butyrate following 0, 1, and 3 mcg/kg GH, whereas there were no differences between the responses following 3 and 6 mcg/kg GH [R].These dosages were administered intravenously. 1 mg of Somatropin corresponds to 3 IU (International Units) of Somatropin [R].So, this means that for someone who weighs 100 kg, lipolysis is maxed out at about 300 mcg of Somatropin, which is 0.9 IU’s of pharma grade GH IV. We need to math out the corresponding subcutaneous dose based on the bioavailability and bioactivity comparison data we have on humans administered HGH. Obviously IV dosages are not representative of what dosages would be via the far more realistic and tolerable method of administration (subcutaneous). In one study, the mean estimated availability of subcutaneous injected HGH was shown to be 63% of that of HGH administered I.V. after correcting for differences in the GH dose [R].Another study found the availability of subcutaneous injected HGH to be about 70% of that of HGH administered I.V. [R].So, for a 100 kg man, fat loss benefits would be maxed out around 1.35 IU of GH per administration subcutaneously. There is a refractory period cells need before another pulse of exogenous GH can even be useful in a lipolysis context, which I will delve into further in a future article. Related

At supraphysiological dosages, taking GH before going to sleep makes sense because there is a negative feedback loop on the growth hormone (GH)/insulin-like growth factor I (IGF-1) axis.In this study, IGF-1 was constantly infused, and the effect on endogenous GH secretion was assessed [R].Overall, GH concentrations were suppressed during the rhIGF-I infusion by 85±3%, mainly by attenuating spontaneous GH pulse amplitude (77±4% suppression).The apparent GH pulse frequency was attenuated from 7.8±0.9 to 4.7±0.6 pulses/24h (P= 0.006).IGF-1 is the primary feedback, and the mechanism is similar to what you see with the HPTA axis.When there is enough Testosterone and Estrogen, your body has this mechanism in place to prevent you from making more.The same thing happens with exogenous GH use.When you administer exogenous GH, IGF-1 elevates.When your body recognizes that IGF-1 is elevated, it starts to suppress GH pulse amplitude in a dose dependent manner.If you are using supraphysiological amounts of GH for bodybuilding purposes, you can assume that your natural secretion is greatly inhibited during sleep as this negative feedback loop will prevent your body from secreting GH in your sleep like it normally would.When endogenous GH production is suppressed, administering exogenous GH at times that lipolysis or muscle growth can be optimized is the most logical approach to injection timing.[embedded content]If fat loss is the goal, then taking GH in a fasted state prior to fasted cardio would be ideal.There is a ceiling on how much GH is needed to maximize lipolysis, so if your dosage exceeds 2 IU per day, then I would advise splitting up that dose into two (or more if needed) different periods of the day with as much time between them as possible.If muscle growth is the goal, GH should still be administered in a pulsatile fashion to mimic endogenous secretions.Topped out stimulation of autocrine IGF-1 in skeletal muscle can be achieved with fairly low dosages of exogenous GH, and administering a higher dose above and beyond that may just be a waste.This is why it would be wise to split up your daily GH dose into multiple administrations if you are using supraphysiological dosages.Related