Pristi1340
New member
Can you PM it to me??? If you don't want to I understand man
i didn't save the email. just PM him sometime
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Can you PM it to me??? If you don't want to I understand man
I am not going to discount what others have said, as I do not know how much you are trying to filter nor how quickly. I used bottle top once, about a decade ago, and it was nightmare. Personally, as soon as individual is ready to graduate from syringe filters, I feel they should go directly to peristaltic pump and whatman polycap filter.
For pump, get this one:http://www.novatech-usa.com/Products/Dolphin-Peristaltic-Pumps-Parts/UD75-XA-LSAUXXX
UD75-XA-LSAUXXX
MEC-O-MATIC Dolphin Series Peristaltic Metering Pump
97 GPD @ 25 PSI, 115 VAC
(Manual Run Time Control)
If you only purchase one, it will cost you $240.37.
The first time you use it, you will know you got your money's worth.
Used in conjunction with Whatman Polycap 75AS, 0.2um, with Filling Bell, you can filter approximately 255ml/min. Or 1 Liter of media every 4 minutes.
I recommend using an autoclaved 2000ml Hybex media bottle.
Once you are proficient, you will be able to throw Polycap onto Hybex bottle and start up pump, then occupy yourself with other lab tasks for entire 8 minutes it takes to filter 2L of media into Hybex bottle, stop pump and cap bottle, move filter onto another Hybex and crank it up again.
Once all of your media has been filtered, use a bottle top dispenser to fill vials. Even manual dispenser and you should be able to run 50-75/min.
Sorry but I oppose all systems that are not closed loop. Look for a whatman or millipor 90mm .22u in line filter. Can do many liters. Use gentle heat and get a vacuum pump.
Hello I am new here but not new to the world of this industry. I have tried the bottle top millipore and nalgen system .22 500/1000ml systems with a vacuum pump. Took way too long and had a few break although they did filter very effectively. Efficiency is what I'm more concerned with now for sterility purpose on a larger scale but can't afford for a 3hr 500ml system anymore. The inline system you describe sounds like the way to go as long as it is .22 can you point me in a direction? I have been looking but am trying to set up a correct and exact system.
Any help would be greatly appreciated thank you
Sorry to ressurect such an old thread but theres a lot of good info ive found in here. Ive been trying to find alternatives to the shitty filtertop units that either crack, clog, or just fuck up in some way.
6-fingers had some really good info on using a perastaltic pump along with a whatman polycap filter. Is there anyone else here that uses this set up? Pretty sure i have a general idea of how things would work but if someone could explain in a little more detail that would be great. Also, i realize this was posted 4 years ago so if there are any other preferred ways for filtering im open to suggestions.
If you find the right manufacturer you won't have those problems. The actual bottle top filter has never cracked when I've used them, is that even possible?
If you're talking about the plastic bottle, use glass media bottle instead. Plastic bottles crack 90% of the time, they aren't made to handle that kind of pressure for that long.
Polycaps are used for filtering very large amounts.
I always recommend regular plastic bottle top filters (just check if it's compatible with the solvents you're using and look around what other people are using)
The reason why I recommend them is because they are very convenient and cheap. You don't have to sterilize them and all that.
If you've already decided to use one, you pretty much need sterile tubing for the pumphead, a pumphead,a controlunit/or just a pump and ofcourse the actual filter.
If you could be more specifik I could try to answer your question
U always need glass media bottles.With the bottle top filters, yes i was talking about the recievers cracking. I have switched to glass media bottles, but the filters still end up clogging and dripping very slowly, if at all. I recently used a millipore filter unit and it stopped dripping after 50ml and leeched a blue pigment from the plastic into my brew. If you dont mind me asking, what brand filters are you using? I am doing decent amounts of volume with multiple compounds.
What does a pumphead look like? I only thought id need the pump with tubing and a polycap filter. Are there any other ways to use a polycap filter? I can't find much about them online. Thanks for your reply.
With regular solvents and co-solvents at normal % (BA,BB) you can use PVDF filter or Nylon sometimes
but if you brew with EO or GUA (no matter what % on it) you must use PTFE only
If you are safety player just use PTFE membranes only,but it's slower but safer than other membranes
The bigger volume guys use Whatman capsule with peristaltic pump but it will leave behind around 40ml. Electric vac pump will pull that thru.Thanks for that info. Do you have any experience with using a pump like the one i posted a few posts back? I held off on ordering it until i find out more info. All i know is i am sick of these disposable filters. I need to get something thats all glass and autoclaveable. Doesnt anyone use other set ups than these cheap plastic filters?